Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS
文献类型：期刊论文
| 作者 | Yu, Tian1,2; Xu, Hang3; Wang, Weiwen3 ; Li, Shifei1,2; Chen, Zheng1,2; Deng, Huihua1,2,3
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2015-10-01 |
| 卷号 | 1002期号:0页码:267-276 |
| 通讯作者邮箱 | dengrcls@seu.edu.cn (H. Deng). |
| 关键词 | Hair corticosterone Blood corticosterone Brain corticosterone Repeated social defeat Atmospheric pressure chemical ionization |
| ISSN号 | 1570-0232 |
| 通讯作者 | Deng, Huihua |
| 英文摘要 | Endogenous corticosterone in rodent's hair would be a potential biomarker to assess the response of the hypothalamus-pituitary-adrenal axis to chronic stress. However, currently unknown is whether hair corticosterone is associated with endogenous corticosterone in blood and brain. The present study aimed to develop an enhanced assay for determination of endogenous corticosterone in blood, brain and hair, and to examine associations of hair corticosterone with blood and brain corticosterone under basal condition and association with blood corticosterone under chronic stressful condition. Hair at the back and blood samples were collected from non-stressed and stressed rodents, and prefrontal lobe and thalamus from non-stressed rodents. Chronic stress exerted on mice was 30-day repeated social defeat. The analyses were done using high performance liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization in positive mode. Limits of detection and quantification were 0.2 and 0.5 ng/ml for rat's blood, and 0.5 and 1.0 pg/mg for rat's hair and brain, and 1.25 and 2.50 ng/ml (or pg/mg) for mouse's blood (or hair). Recovery ranged from 84.2 to 108.0%. The intra- and inter-day coefficients of variation were less than 10%. Additionally, correlation of hair corticosterone with blood corticosterone was significant in both mice and rats, but correlations with corticosterone in prefrontal lobe and thalamus were not significant in rats. Both hair and blood corticosterone were significantly higher in stressed mice compared with controls. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 项目简介 | We wish to thank Prof. Wei Lu and Miss Yuehua Zhu (Key Laboratory of Development Genes and Human Diseases, Ministry of Education, Southeast University) for their assistance in rats' sampling. This research was supported by Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Science, and in part by the National Basic Research Program of China under Grant 2015CB351704, and by the Fundamental Research Funds for the Southeast University, and by Jiangsu Provincial Innovation Project for Scientific Research of Graduate Students in Universities (KYZZ_0069), China. |
| 语种 | 英语 |
| WOS记录号 | WOS:000362132100034 |
| 源URL | [http://ir.psych.ac.cn/handle/311026/15266]  |
| 专题 | 心理研究所_中国科学院心理健康重点实验室 |
| 作者单位 | 1.Southeast Univ, Key Lab Child Dev & Learning Sci, Minist Educ, Nanjing 210096, Jiangsu, Peoples R China 2.Southeast Univ, Inst Child Dev & Educ, Res Ctr Learning Sci, Nanjing 210096, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Tian,Xu, Hang,Wang, Weiwen,et al. Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2015,1002(0):267-276. |
| APA | Yu, Tian,Xu, Hang,Wang, Weiwen,Li, Shifei,Chen, Zheng,&Deng, Huihua.(2015).Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1002(0),267-276. |
| MLA | Yu, Tian,et al."Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1002.0(2015):267-276. |
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来源：心理研究所
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