Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116
文献类型：期刊论文
| 作者 | Gao, Mang1; Song, Xiaojin1; Feng, Yingang1; Li, Wenli3; Cui, Qiu1,2 |
| 刊名 | JOURNAL OF OLEO SCIENCE
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 62期号:3页码:143-151 |
| 关键词 | Aurantiochytrium sp. biomass docosahexaenoic acid heterotrophic marine algae lipid |
| 英文摘要 | A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis of the 18S rDNA of SD116 showed that the strain has a close phylogenetic relationship to Aurantiochytrium species. The highest rates for growth and DHA accumulation for SD116 were obtained in 6.0% glucose, 2.0% yeast extract, and 50% artificial seawater (ASW) at a pH of 7 at 28 degrees C. The maximum total lipid content reached 56.3% of the dry cell weight (DCW), and the maximum DHA content accounted for 50.9% of the total fatty acid (TFA) content. It was further found that urea may be a potential nitrogen source for industrial fermentation because of its cheap price and ability to induce a relatively high biomass and lipid production capacity. Using 5 L fermenters, the DCW, total lipid content, and DHA yield were found to be 70.43 g L-1, 71.09% of the DCW, and 17.42 g L-1 (34.79% of the TFA), respectively. The results show that Aurantiochytrium sp. SD116 is a promising candidate for commercial DHA production and could be useful for the synthesis of biomass-related products. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| 类目[WOS] | Chemistry, Applied ; Food Science & Technology |
| 研究领域[WOS] | Chemistry ; Food Science & Technology |
| 关键词[WOS] | CARDIAC SUDDEN-DEATH ; SCHIZOCHYTRIUM-LIMACINUM ; CULTURE-CONDITIONS ; FATTY-ACIDS ; OPTIMIZATION ; GROWTH ; BIOSYNTHESIS ; PREVENTION ; MANGROVEI |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315362000004 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/5949]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 3.Ocean Univ China, Key Lab Marine Drugs, Minist Educ, Sch Med & Pharm, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Mang,Song, Xiaojin,Feng, Yingang,et al. Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116[J]. JOURNAL OF OLEO SCIENCE,2013,62(3):143-151. |
| APA | Gao, Mang,Song, Xiaojin,Feng, Yingang,Li, Wenli,&Cui, Qiu.(2013).Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116.JOURNAL OF OLEO SCIENCE,62(3),143-151. |
| MLA | Gao, Mang,et al."Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116".JOURNAL OF OLEO SCIENCE 62.3(2013):143-151. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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