Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold
文献类型：期刊论文
| 作者 | Feng, Yingang1 ; Song, Xiaxia2; Lin, Jinzhong3; Xuan, Jinsong2; Cui, Qiu1; Wang, Jinfeng3
|
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2014-07-18 |
| 卷号 | 450期号:1页码:67-72 |
| 关键词 | Ribosomal protein Protein structure Archaea NMR Protein fold |
| 英文摘要 | Three archaea-specific ribosomal proteins recently identified show no sequence homology with other known proteins. Here we determined the structure of L46a, the most conserved one among the three proteins, from Sulfolobus solfataricus P2 using NMR spectroscopy. The structure presents a twisted beta-sheet formed by the N-terminal part and two helices at the C-terminus. The L46a structure has a positively charged surface which is conserved in the L46a protein family and is the potential rRNA-binding site. Searching homologous structures in Protein Data Bank revealed that the structure of L46a represents a novel protein fold. The backbone dynamics identified by NMR relaxation experiments reveal significant flexibility at the rRNA binding surface. The potential position of L46a on the ribosome was proposed by fitting the structure into a previous electron microscopy map of the ribosomal 50S subunit, which indicated that L46a contacts to domain I of 23S rRNA near a multifunctional ribosomal protein L7ae. (C) 2014 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BACKBONE DYNAMICS ; NOE ASSIGNMENT ; NMR ; RELAXATION ; DATABASE ; PROGRAM ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343641000012 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6129]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Dept Biol Sci & Engn, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Yingang,Song, Xiaxia,Lin, Jinzhong,et al. Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,450(1):67-72. |
| APA | Feng, Yingang,Song, Xiaxia,Lin, Jinzhong,Xuan, Jinsong,Cui, Qiu,&Wang, Jinfeng.(2014).Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,450(1),67-72. |
| MLA | Feng, Yingang,et al."Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 450.1(2014):67-72. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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