Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation
文献类型：期刊论文
| 作者 | Zheng, Sanduo1,2; Villa, Raffaella3; Wang, Jia1,2; Feng, Yingang4,5 ; Wang, Jinfeng6; Becker, Peter B.3; Ye, Keqiong2,7
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| 刊名 | GENES & DEVELOPMENT
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 28期号:23页码:2652-2662 |
| 关键词 | dosage compensation crystal structure DNA-protein complex nonspecific DNA binding |
| 英文摘要 | The male-specific lethal dosage compensation complex (MSL-DCC) selectively assembles on the X chromosome in Drosophila males and activates gene transcription by twofold through histone acetylation. An MSL recognition element (MRE) sequence motif nucleates the initial MSL association, but how it is recognized remains unknown. Here, we identified the CXC domain of MSL2 specifically recognizing the MRE motif and determined its crystal structure bound to specific and nonspecific DNAs. The CXC domain primarily contacts one strand of DNA duplex and employs a single arginine to directly read out dinucleotide sequences from the minor groove. The arginine is flexible when bound to nonspecific sequences. The core region of the MRE motif harbors two binding sites on opposite strands that can cooperatively recruit a CXC dimer. Specific DNA-binding mutants of MSL2 are impaired in MRE binding and X chromosome localization in vivo. Our results reveal multiple dynamic DNA-binding modes of the CXC domain that target the MSL-DCC to X chromosomes. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Cell Biology ; Developmental Biology ; Genetics & Heredity |
| 研究领域[WOS] | Cell Biology ; Developmental Biology ; Genetics & Heredity |
| 关键词[WOS] | HISTONE ACETYLTRANSFERASE ; SEX-CHROMOSOMES ; RING FINGER ; ROX RNAS ; COMPLEX ; PROTEINS ; MOF ; ASSOCIATION ; ACETYLATION ; RECRUITMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345812000008 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6320]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 作者单位 | 1.Beijing Normal Univ, Dept Biochem & Mol Biol, Coll Life Sci, Beijing 100875, Peoples R China 2.Natl Inst Biol Sci, Beijing 102206, Peoples R China 3.Univ Munich, Mol Biol Unit, Adolf Butenandt Inst, Ctr Integrated Prot Sci, D-80336 Munich, Germany 4.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Shangdong, Peoples R China 5.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shangdong, Peoples R China 6.Chinese Acad Sci, Natl Lab Biomacromol, Beijing 100101, Peoples R China 7.Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Sanduo,Villa, Raffaella,Wang, Jia,et al. Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation[J]. GENES & DEVELOPMENT,2014,28(23):2652-2662. |
| APA | Zheng, Sanduo.,Villa, Raffaella.,Wang, Jia.,Feng, Yingang.,Wang, Jinfeng.,...&Ye, Keqiong.(2014).Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation.GENES & DEVELOPMENT,28(23),2652-2662. |
| MLA | Zheng, Sanduo,et al."Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation".GENES & DEVELOPMENT 28.23(2014):2652-2662. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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