Raman Activated Cell Ejection for Isolation of Single Cells
文献类型：期刊论文
| 作者 | Wang, Yun1,2 ; Ji, Yuetong1,2; Wharfe, Emma S.3; Meadows, Roger S.4; March, Peter4; Goodacre, Royston5; Xu, Jian1,2; Huang, Wei E.3
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2013-11-19 |
| 卷号 | 85期号:22页码:10697-10701 |
| 英文摘要 | We have optimized a Raman microscope to obtain a single cell Raman spectrum (SCRS) with 0.1 s acquisition time. SCRS with such short acquisition time has sufficient discriminatory ability and spectral reproducibility to differentiate cells incorporated with C-13 and N-15 and to classify five different types of bacteria isolated from the oral cavity. We also developed Raman activated cell ejection (RACE) that is assisted by laser induced forward transfer (LIFT). We have shown, for the first time, that the single cells of interest can be identified and then accurately isolated from complex microbial communities based on their SCRS. This approach can be used to sort single cells of target traits from complex samples (e.g., biofilms, soils, sludge, tissues). |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | IN-SITU HYBRIDIZATION ; SPECTROSCOPY ; MICROSPECTROSCOPY ; BACTERIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327360900013 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/5948]  |
| 专题 | 青岛生物能源与过程研究所_单细胞中心 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 3.Univ Sheffield, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England 4.Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England 5.Univ Manchester, Manchester Inst Biotechnol, Sch Chem, Manchester M1 7DN, Lancs, England |
| 推荐引用方式 GB/T 7714 | Wang, Yun,Ji, Yuetong,Wharfe, Emma S.,et al. Raman Activated Cell Ejection for Isolation of Single Cells[J]. ANALYTICAL CHEMISTRY,2013,85(22):10697-10701. |
| APA | Wang, Yun.,Ji, Yuetong.,Wharfe, Emma S..,Meadows, Roger S..,March, Peter.,...&Huang, Wei E..(2013).Raman Activated Cell Ejection for Isolation of Single Cells.ANALYTICAL CHEMISTRY,85(22),10697-10701. |
| MLA | Wang, Yun,et al."Raman Activated Cell Ejection for Isolation of Single Cells".ANALYTICAL CHEMISTRY 85.22(2013):10697-10701. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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