Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses
文献类型：期刊论文
| 作者 | Liu, Wu-Yi1,2; Zhang, Ke-Jun3 |
| 刊名 | ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 6期号:9页码:958-965 |
| 关键词 | Toad and frog salt-extraction genomic DNA formalin PCR amplification |
| 英文摘要 | The technical procedures for extraction of DNA from formalin-fixed tissues include many steps such as chemical treatment, enzymatic digestion, phenol-chloroform purification and alcohol precipitation. Formalin-fixed specimens used in molecular cell and DNA studies have shown shortcomings with respect to the efficacy of DNA isolation and subsequent PCR (Polymerase Chain Reaction) amplification. This study was designed to simplify and maximize recovery of PCR-amplifiable DNA from formalin-fixed toad and frog specimens and also to minimize co-extraction of substances that inhibit PCR amplification. This is achieved by a combination of DNA extraction from formalin-fixed muscle tissues using a salt-out buffer consisting of EDTA and proteinase K and NaCl. All steps are performed at room temperature (20-25 degrees C), thereby reducing further degradation of the already damaged fragile specimen DNA and providing an optimal trade-off between DNA release and degradation. The salt-extraction method of genomic DNA presented here allows DNA isolation from formalin-fixed tissues with a minimum of working steps and equipment and rapidly yields much DNA. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Veterinary Sciences |
| 研究领域[WOS] | Veterinary Sciences |
| 关键词[WOS] | PARAFFIN-EMBEDDED TISSUES ; POLYMERASE-CHAIN-REACTION ; GENE-EXPRESSION ; REACTION AMPLIFICATION ; BUCCAL CELLS ; SECTIONS ; RETRIEVAL ; SAMPLES ; OPTIMIZATION ; COLLECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000294653200009 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6549]  |
| 专题 | 青岛生物能源与过程研究所_仿生能源与储能系统团队 |
| 作者单位 | 1.Fuyang Normal Coll, Dept Biol Sci, Fuyang City 236041, Peoples R China 2.Fuyang Normal Coll, Dept Sci Res, Fuyang City 236041, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Biomass Energy & Bioproc Technol, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Wu-Yi,Zhang, Ke-Jun. Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses[J]. ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES,2011,6(9):958-965. |
| APA | Liu, Wu-Yi,&Zhang, Ke-Jun.(2011).Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses.ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES,6(9),958-965. |
| MLA | Liu, Wu-Yi,et al."Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses".ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES 6.9(2011):958-965. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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