Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues
文献类型：期刊论文
| 作者 | Zhang, Dianbao1,2; Wang, Jing3; Wang, Zhe4; Wang, Rui1,2; Song, Lina5; Zhang, Tao1,2; Lin, Xuewen1,2; Shi, Ping6; Xin, Hongchuan7; Pang, Xining1,2 |
| 刊名 | SCIENCE OF ADVANCED MATERIALS
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 7期号:6页码:1058-1064 |
| 关键词 | PEI-Coated Fe3O4 Nanoparticles Mesenchymal Stem Cells siRNA Delivery Endocytosis |
| 英文摘要 | Mesenchymal stem cells (MSCs) are emerging as a promising therapeutic approach and genetic modification by non-viral vectors could span their applications. Herein, the capacity of PEI-coated Fe3O4 nanoparticles (NPs) for siRNA delivery to human MSCs derived from different tissues was evaluated. The prepared spherical NPs with charge of around +44.0 mV and an average diameter of 12 nm exhibited proper siRNA encapsulation and enhanced serum stability. High-purity human MSCs were isolated from bone marrow, adipose tissue and amnion membrane, and GAPDH siRNA and siRNA NC (negative control) was transfected into MSCs using NPs prepared. When 4 ng/ml NPs were used at NPs/siRNA weight ratio of 4, high transfection efficiency and low toxicity were observed and silencing efficiencies were higher than 60%. Additionally, the in vitro tests indicated that the NPs entered into MSCs by endocytosis. According to those results, PEI-coated Fe3O4 NPs possessed potential as a safe and effective siRNA delivery reagent for MSCs and may be fit for stem cell therapy. |
| WOS标题词 | Science & Technology ; Technology ; Physical Sciences |
| 类目[WOS] | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science ; Physics |
| 关键词[WOS] | IRON-OXIDE NANOPARTICLES ; GENE DELIVERY ; MEDICINE ; TRANSFECTION ; THERAPY ; DISEASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352332800007 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6423]  |
| 专题 | 青岛生物能源与过程研究所_科技发展部 |
| 作者单位 | 1.China Med Univ, Minist Publ Hlth, Key Lab Cell Biol, Dept Stem Cells & Regenerat Med, Shenyang 110001, Peoples R China 2.China Med Univ, Minist Educ, Key Lab Med Cell Biol, Shenyang 110001, Peoples R China 3.China Med Univ, Affiliated Hosp 1, Dept Anus & Intestine Surg, Shenyang 110001, Peoples R China 4.China Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China 5.Southeast Univ, Sch Biol Sci & Med Engn, Jiangsu Lab Biomat & Devices, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China 6.China Med Univ, Affiliated Hosp 1, Dept Gen Practice, Shenyang 110001, Peoples R China 7.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dianbao,Wang, Jing,Wang, Zhe,et al. Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues[J]. SCIENCE OF ADVANCED MATERIALS,2015,7(6):1058-1064. |
| APA | Zhang, Dianbao.,Wang, Jing.,Wang, Zhe.,Wang, Rui.,Song, Lina.,...&Pang, Xining.(2015).Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues.SCIENCE OF ADVANCED MATERIALS,7(6),1058-1064. |
| MLA | Zhang, Dianbao,et al."Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues".SCIENCE OF ADVANCED MATERIALS 7.6(2015):1058-1064. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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