Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid
文献类型：期刊论文
| 作者 | Zhang, Wei1; Cao, Shaona1; Qiu, Li2; Qi, Fengxia1 ; Li, Zhong1,3; Yang, Ying1; Huang, Shaohua1; Bai, Fali1; Liu, Changning4; Wan, Xiaobo2
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| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2015-03-02 |
| 卷号 | 16期号:4页码:565-569 |
| 关键词 | biosynthesis methyltransferases mycophenolic acid substrate flexibility transferases |
| 英文摘要 | Mycophenolic acid (MPA, 1) is a clinically important immunosuppressant. In this report, a gene cluster mpa responsible for the biosynthesis of 1 was identified from Penicillium brevicompactum NRRL 864. The S-adenosyl-L-methionine-dependent (SAM-dependent) O-methyltransferase encoded by the mpaG gene was functionally and kinetically characterized in vitro. MpaG catalyzes the methylation of demethylmycophenolic acid (DMMPA, 6) to form 1. It also showed significant substrate flexibility by methylating two structural derivatives of 6 prepared by organic synthesis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Medicinal |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | PENICILLIUM-BREVICOMPACTUM ; DEHYDROGENASE ; FUNGI |
| 收录类别 | SCI ; IC |
| 语种 | 英语 |
| WOS记录号 | WOS:000350138700005 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6083]  |
| 专题 | 青岛生物能源与过程研究所_酶工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci Menglun, Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei,Cao, Shaona,Qiu, Li,et al. Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid[J]. CHEMBIOCHEM,2015,16(4):565-569. |
| APA | Zhang, Wei.,Cao, Shaona.,Qiu, Li.,Qi, Fengxia.,Li, Zhong.,...&Li, Shengying.(2015).Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid.CHEMBIOCHEM,16(4),565-569. |
| MLA | Zhang, Wei,et al."Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid".CHEMBIOCHEM 16.4(2015):565-569. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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