Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair
文献类型：期刊论文
| 作者 | Li, Chongjie1; Ma, Li2,3; Mou, Shanli4; Wang, Yibin1; Zheng, Zhou1; Liu, Fangming1; Qi, Xiaoqing1; An, Meiling1; Chen, Hao1; Miao, Jinlai1,5 |
| 刊名 | MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 773页码:37-42 |
| 关键词 | Chlamydomonas sp ICE-L UV Photolyase Photo-reactivation |
| 英文摘要 | Bacteria living in the Antarctic region have developed several adaptive features for growth and survival under extreme conditions. Chlamydomonas sp. ICE-Lis well adapted to high levels of solar UV radiation. A putative photolyase was identified in the Chlamydomonas sp. ICE-L transcriptome. The complete cDNA sequence was obtained by RACE-PCR. This PHR encoding includes a polypeptide of 579 amino acids with clear photolyase signatures belonging to class II CPD-photolyases, sharing a high degree of homology with Chlamydomonas reinhardtii (68%). Real-time PCR was performed to investigate the potential DNA damage and responses following UVB exposure. CPD photolyase mRNA expression level increased over 50-fold in response to UVB radiation for 6 h. Using photolyase complementation assay, we demonstrated that DNA photolyase increased photo-repair more than 116-fold in Escherichia coil strain SY2 under 100 mu w/cm(2) UVB radiation. To determine whether photolyase is active in vitro, CPD photolyase was over-expressed. It was shown that pyrimidine dimers were split by the action of PHR2. This study reports the unique structure and high activity of the enzyme. These findings are relevant for further understanding of molecular mechanisms of photo-reactivation, and will accelerate the utilization of photolyase in the medical field. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology |
| 关键词[WOS] | SP ICE-L ; HUMAN SKIN ; EXPRESSION ; APOPTOSIS ; STRESS ; PHOTOPROTECTION ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351979200006 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6415]  |
| 专题 | 青岛生物能源与过程研究所_酶工程团队 |
| 作者单位 | 1.State Ocean Adm, Key Lab Marine Bioact Subst, Inst Oceanog 1, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China 5.State Key Lab Biol Fermentat Engn Beer Preparat, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chongjie,Ma, Li,Mou, Shanli,et al. Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2015,773:37-42. |
| APA | Li, Chongjie.,Ma, Li.,Mou, Shanli.,Wang, Yibin.,Zheng, Zhou.,...&Miao, Jinlai.(2015).Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,773,37-42. |
| MLA | Li, Chongjie,et al."Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 773(2015):37-42. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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