Specific Probe Selection from Landscape Phage Display Library and Its Application in Enzyme-Linked Immunosorbent Assay of Free Prostate-Specific Antigen
文献类型：期刊论文
| 作者 | Lang, Qiaolin1,2 ; Wang, Fei1,2,3; Yin, Long1,2,3; Liu, Mingjun4; Petrenko, Valery A.5; Liu, Aihua1,2,3
|
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014-03-04 |
| 卷号 | 86期号:5页码:2767-2774 |
| 英文摘要 | Probes against targets can be selected from the landscape phage library f8/8, displaying random octapeptides on the pVIII coat protein of the phage fd-tet and demonstrating many excellent features including multivalency, stability, and high structural homogeneity. Prostate-specific antigen (PSA) is usually determined by immunoassay, by which antibodies are frequently used as the specific probes. Herein we found that more advanced probes against free prostate-specific antigen (f-PSA) can be screened from the landscape phage library. Four phage monoclones were selected and identified by the specificity array. One phage clone displaying the fusion peptide ERNSVSPS showed good specificity and affinity to f-PSA and was used as a PSA capture probe in a sandwich enzyme-linked immunosorbent assay (ELISA) array. An anti-human PSA monoclonal antibody (anti-PSA mAb) was used to recognize the captured antigen, followed by horseradish peroxidase-conjugated antibody (HRP-IgG) and o-phenylenediamine, which were successively added to develop plate color. The ELISA conditions such as effect of blocking agent, coating buffer pH, phage concentration, antigen incubation time, and anti-PSA mAb dilution for phage ELISA were optimized. On the basis of the optimal phage ELISA conditions, the absorbance taken at 492 nm on a microplate reader was linear with f-PSA concentration within 0.825 similar to 165 ng/mL with a low limit of detection of 0.16 ng/mL. Thus, the landscape phage is an attractive biomolecular probe in bioanalysis. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | RESOLVED IMMUNOFLUOROMETRIC ASSAY ; BIOCHEMICAL CHARACTERISTICS ; FILAMENTOUS BACTERIOPHAGE ; SALMONELLA-TYPHIMURIUM ; CANCER-DETECTION ; PEPTIDE LIBRARY ; MOLECULAR-FORMS ; TOTAL PSA ; SURFACE ; VIRUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332494100068 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6191]  |
| 专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Univ, Affiliated Hosp, Coll Med, Dept Clin Lab, Qingdao 266003, Peoples R China 5.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA |
| 推荐引用方式 GB/T 7714 | Lang, Qiaolin,Wang, Fei,Yin, Long,et al. Specific Probe Selection from Landscape Phage Display Library and Its Application in Enzyme-Linked Immunosorbent Assay of Free Prostate-Specific Antigen[J]. ANALYTICAL CHEMISTRY,2014,86(5):2767-2774. |
| APA | Lang, Qiaolin,Wang, Fei,Yin, Long,Liu, Mingjun,Petrenko, Valery A.,&Liu, Aihua.(2014).Specific Probe Selection from Landscape Phage Display Library and Its Application in Enzyme-Linked Immunosorbent Assay of Free Prostate-Specific Antigen.ANALYTICAL CHEMISTRY,86(5),2767-2774. |
| MLA | Lang, Qiaolin,et al."Specific Probe Selection from Landscape Phage Display Library and Its Application in Enzyme-Linked Immunosorbent Assay of Free Prostate-Specific Antigen".ANALYTICAL CHEMISTRY 86.5(2014):2767-2774. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。