Sensitive electrochemical microbial biosensor for p-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons
文献类型：期刊论文
| 作者 | Tang, Xiangjiang1,2,3 ; Zhang, Tingting1,2,3,4; Liang, Bo1,2,3; Han, Dongfei1,2,3; Zeng, Lingxing5; Zheng, Cheng5; Li, Tie4; Wei, Mingdeng5; Liu, Aihua1,2,3
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2014-10-15 |
| 卷号 | 60页码:137-142 |
| 关键词 | Bacterial surface display Organophosphorus hydrolase p-nitrophenyl organophosphates Ordered mesoporous carbons Electrochemical microbial biosensor Amperometric detection |
| 英文摘要 | A novel electrochemical microbial biosensor for the rapid monitoring of p-nitrophenyl-substituted organophosphates (OPs) compounds based on glass carbon electrode (GCE) modified with both ordered mesopore carbons (OMCs) and cell surface-expressed organophosphorus hydrolase (OPH) (OPH-bacteria/ OMCs/GCE) was described in this paper. The genetically engineered Escherichia coli strain surface displayed mutant OPH (55) with improved enzyme activity and favorable stability was constructed using a newly identified N-terminal of ice nucleation protein as an anchoring motif, which can be used directly without further time-consuming enzyme-extraction and purification, thereafter greatly improved the stability of the enzyme. Compared to OPH-bacteria modified GCE (OPH-bacteria/GCE), the OPH-bacteria/OMCs/GCE not only significantly enhanced the current response but also reduced the oxidation overpotential towards oxidizable p-nitrophenol (p-NP), which was the hydrolysate of p-nitrophenyl-substituted OPs. Under the optimized experimental conditions, at +0.84 V (vs. SCE), the current-time curve was performed with varying OPs concentration. The current response was linear with paraoxon concentration within 0.05-25 mu M. Similarly, linear range of 0.05-25 mu M was found for parathion, and 0.08-30 mu M for methyl parathion. The low limits of detection were evaluated to be 9.0 nM for paraoxon, 10 nM for parathion and 15 nM for methyl parathion (S/N=3). Thus, a highly specific, sensitive and rapid microbial biosensor was established, which holds great promise for on-site detection of trace p-nitrophenyl-substituted OPs. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | NERVE AGENTS ; WHOLE-CELL ; D-XYLOSE ; PESTICIDES ; PARAOXON ; SAMPLES ; SYSTEM ; SENSOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337863900019 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6270]  |
| 专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
| 作者单位 | 1.Chinese Acad Sci, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China 5.Fuzhou Univ, Inst Adv Energy Mat, Fuzhou 350002, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Xiangjiang,Zhang, Tingting,Liang, Bo,et al. Sensitive electrochemical microbial biosensor for p-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons[J]. BIOSENSORS & BIOELECTRONICS,2014,60:137-142. |
| APA | Tang, Xiangjiang.,Zhang, Tingting.,Liang, Bo.,Han, Dongfei.,Zeng, Lingxing.,...&Liu, Aihua.(2014).Sensitive electrochemical microbial biosensor for p-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons.BIOSENSORS & BIOELECTRONICS,60,137-142. |
| MLA | Tang, Xiangjiang,et al."Sensitive electrochemical microbial biosensor for p-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons".BIOSENSORS & BIOELECTRONICS 60(2014):137-142. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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