Specific ligands for classical swine fever virus screened from landscape phage display library
文献类型：期刊论文
| 作者 | Yin, Long1,2,3; Luo, Yuzi1; Liang, Bo2 ; Wang, Fei2,3; Du, Min1; Petrenko, Valery A.4; Qiu, Hua-Ji1; Liu, Aihua2,3
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| 刊名 | ANTIVIRAL RESEARCH
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 109页码:68-71 |
| 关键词 | Classical swine fever virus E2 protein Phage display Specific ligand Landscape phage display library Antiviral activity |
| 英文摘要 | Classical swine fever (CSF) is a devastating infectious disease caused by classical swine fever virus (CSFV). The screening of CSFV-specific ligands is of great significance for diagnosis and treatment of CSF. Affinity selection from random peptide libraries is an efficient approach to discover ligands with high stability and specificity. Here, we screened phage ligands for the CSFV E2 protein from f8/8 landscape phage display library by biopanning and obtained four phage clones specific for the E2 protein of CSFV. Viral blocking assays indicated that the phage clone displaying the octapeptide sequence DRATSSNA remarkably inhibited the CSFV replication in PK-15 cells at a titer of 10(10) transduction units, as evidenced by significantly decreased viral RNA copies and viral titers. The phage-displayed E2-binding peptides have the potential to be developed as antivirals for CSF. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pharmacology & Pharmacy ; Virology |
| 研究领域[WOS] | Pharmacology & Pharmacy ; Virology |
| 关键词[WOS] | ANTIGEN-SPECIFIC RESIDUES ; PROTEINS E-RNS ; E2 GLYCOPROTEIN ; PEPTIDE LIBRARY ; IDENTIFICATION ; PESTIVIRUSES ; VIRULENCE ; RECEPTORS ; SELECTION ; EPITOPE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341470700009 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6315]  |
| 专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
| 作者单位 | 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA |
| 推荐引用方式 GB/T 7714 | Yin, Long,Luo, Yuzi,Liang, Bo,et al. Specific ligands for classical swine fever virus screened from landscape phage display library[J]. ANTIVIRAL RESEARCH,2014,109:68-71. |
| APA | Yin, Long.,Luo, Yuzi.,Liang, Bo.,Wang, Fei.,Du, Min.,...&Liu, Aihua.(2014).Specific ligands for classical swine fever virus screened from landscape phage display library.ANTIVIRAL RESEARCH,109,68-71. |
| MLA | Yin, Long,et al."Specific ligands for classical swine fever virus screened from landscape phage display library".ANTIVIRAL RESEARCH 109(2014):68-71. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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