Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain
文献类型：期刊论文
| 作者 | Feng, Xinjun1,2 ; Ding, Yamei3; Xian, Mo1; Xu, Xin1; Zhang, Rubing1; Zhao, Guang1
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| 刊名 | BIORESOURCE TECHNOLOGY
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| 出版日期 | 2014-11-01 |
| 卷号 | 172页码:269-275 |
| 关键词 | D-Lactate Glycerol Engineered Klebsiella pneumoniae strain Bioconversion |
| 英文摘要 | In this study, glycerol was used to produce optically pure D-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, D-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve D-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in D-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1 g/L optically pure D-lactate with a yield of 0.82 g/g glycerol, which represented the highest D-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into D-lactate and high potentiality in utilization of crude glycerol from biodiesel industry. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | D-LACTIC ACID ; RECOMBINANT ESCHERICHIA-COLI ; 3-HYDROXYPROPIONIC ACID ; MICROAEROBIC CONDITIONS ; CRUDE GLYCEROL ; 1,3-PROPANEDIOL ; DEHYDROGENASE ; FERMENTATION ; GENE ; METABOLISM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344113800037 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6263]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Xinjun,Ding, Yamei,Xian, Mo,et al. Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain[J]. BIORESOURCE TECHNOLOGY,2014,172:269-275. |
| APA | Feng, Xinjun,Ding, Yamei,Xian, Mo,Xu, Xin,Zhang, Rubing,&Zhao, Guang.(2014).Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain.BIORESOURCE TECHNOLOGY,172,269-275. |
| MLA | Feng, Xinjun,et al."Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain".BIORESOURCE TECHNOLOGY 172(2014):269-275. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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