Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli
文献类型：期刊论文
| 作者 | Yang, Jianming1; Xian, Mo1 ; Su, Sizheng2; Zhao, Guang1; Nie, Qingjuan3; Jiang, Xinglin1; Zheng, Yanning1; Liu, Wei1
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012-04-27 |
| 卷号 | 7期号:4 |
| 英文摘要 | The depleting petroleum reserve, increasingly severe energy crisis, and global climate change are reigniting enthusiasm for seeking sustainable technologies to replace petroleum as a source of fuel and chemicals. In this paper, the efficiency of the MVA pathway on isoprene production has been improved as follows: firstly, in order to increase MVA production, the source of the "upper pathway'' which contains HMG-CoA synthase, acetyl-CoA acetyltransferase and HMG-CoA reductase to covert acetyl-CoA into MVA has been changed from Saccharomyces cerevisiae to Enterococcus faecalis; secondly, to further enhance the production of MVA and isoprene, a alanine 110 of the mvaS gene has been mutated to a glycine. The final genetic strain YJM25 containing the optimized MVA pathway and isoprene synthase from Populus alba can accumulate isoprene up to 6.3 g/L after 40 h of fed-batch cultivation. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | METHYLERYTHRITOL PHOSPHATE-PATHWAY ; HMG-COA SYNTHASE ; MEVALONATE PATHWAY ; ISOPENTENYL DIPHOSPHATE ; BIOSYNTHESIS ; BACTERIA ; AMORPHA-4,11-DIENE ; INTEGRATION ; BIOREACTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305336000005 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6383]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Ctr Biomat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 2.China Risun Coal Chem Grp Ltd, Beijing Risun Chem Technol Inst, Dept Biochem, Beijing, Peoples R China 3.Qingdao Agr Univ, Foreign Languages Sch, Coll English Off, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jianming,Xian, Mo,Su, Sizheng,et al. Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli[J]. PLOS ONE,2012,7(4). |
| APA | Yang, Jianming.,Xian, Mo.,Su, Sizheng.,Zhao, Guang.,Nie, Qingjuan.,...&Liu, Wei.(2012).Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli.PLOS ONE,7(4). |
| MLA | Yang, Jianming,et al."Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E-coli".PLOS ONE 7.4(2012). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。