Lignocellulose degradation, enzyme production and protein enrichment by Trametes versicolor during solid-state fermentation of corn stover
文献类型：期刊论文
| 作者 | Zhu, YouShuang1; Zhang, HaiBo1,2 ; Zhang, YingLong1,3; Huang, Feng1
|
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011-08-17 |
| 卷号 | 10期号:45页码:9182-9192 |
| 关键词 | Corn stover central composite design laccase ligninolysis Trametes versicolor crude protein |
| 英文摘要 | Microbial conversion of corn stover by white rot fungi has the potential to increase its ligninolysis and nutritional value, thereby transforming it into protein-enriched animal feed. Response surface methodology was applied to optimize conditions for the production of lignocellulolytic enzymes by Trametes versicolor during solid-state fermentation of corn stover, as well as enhance ligninolysis and increase the crude protein content. The effects of an additional carbon source (glucose), copper sulfate (CuSO4) and initial moisture content on lignocellulolytic enzymes, changes in chemical constituents and the crude protein content of corn stover were investigated. T. versicolor produced high laccase, moderate xylanase, and low CMCase activity, whereas neither LiP nor MnP activity was detected. An overall 20-fold increase in laccase activity (45.1 U/g corn stover) was achieved under the optimized conditions. The maximum degradation of lignin and hemicellulose was up to 34.8 and 21.9%, respectively. However, the maximum cellulose loss was less than 10.5%. The crude protein content of the fermented corn stover was doubled under the optimized conditions. Therefore, T. versicolor is a potential organism for laccase production using solid-state fermentation, as well as the simultaneous enhancement of delignification and improvement of the crude protein content in corn stover. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | IN-VITRO DIGESTIBILITY ; RESPONSE-SURFACE METHODOLOGY ; LIGNIN DEGRADATION ; PLEUROTUS-OSTREATUS ; LACCASE PRODUCTION ; WHEAT-STRAW ; OPTIMIZATION ; FUNGI ; BIOCONVERSION ; IMPROVEMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000299734600026 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6556]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266071, Peoples R China 3.Shandong Inst Commerce & Technol, Dept Biotechnol, Jinan 250103, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, YouShuang,Zhang, HaiBo,Zhang, YingLong,et al. Lignocellulose degradation, enzyme production and protein enrichment by Trametes versicolor during solid-state fermentation of corn stover[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(45):9182-9192. |
| APA | Zhu, YouShuang,Zhang, HaiBo,Zhang, YingLong,&Huang, Feng.(2011).Lignocellulose degradation, enzyme production and protein enrichment by Trametes versicolor during solid-state fermentation of corn stover.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(45),9182-9192. |
| MLA | Zhu, YouShuang,et al."Lignocellulose degradation, enzyme production and protein enrichment by Trametes versicolor during solid-state fermentation of corn stover".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.45(2011):9182-9192. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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