The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions
文献类型：期刊论文
| 作者 | Zhang Yinglong1,2; Zhang Haibo1,3 ; Zhu Youshuang1; Huang Feng1
|
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011-11-07 |
| 卷号 | 10期号:69页码:15647-15655 |
| 关键词 | Cellulase-treated lignin laccase [bmim]Cl |
| 英文摘要 | In this study, the effect of laccase earlier obtained by our laboratory on cellulase-treated lignin (CEL) in two different solution systems was further investigated. Results obtained were as follows: After laccase treatment of CEL in the heterogeneous water solution, CEL was then compared with control sample A. Ultraviolet (UV) spectra showed that the total absorbance of sample B increased at 205 and 280 nm; Fourier-transform infrared (FTIR) spectra showed that the carbonyl group obviously appeared; and C-13 nuclear magnetic resonance (NMR) spectra showed that the C-gamma signals disappeared, the C-alpha signals significantly increased, and the methoxyl content decreased. After laccase treatment of CEL in the [bmim]Cl/H2O homogeneous solution, CEL was compared with control sample C. UV spectra showed that the total absorbance of sample D decreased at 280 nm; high-performance liquid chromatography (HPLC) analysis showed that some small absorption peaks disappeared; and C-13 NMR spectra showed that the C-gamma signals obviously increased, the C-alpha signals slightly decreased, while the methoxyl content increased. Clearly, for insoluble CEL in the heterogeneous water solution, laccase partly oxidized the hydroxyl group into a carbonyl group, and thereby partly degraded CEL to increase its solubility. In contrast, for soluble CEL in the [bmim]Cl/H2O homogeneous solution, laccase primarily polymerized small molecule fragments with the CEL macromolecule, increased the methoxyl content of CEL, and thereby played a role in lignin polymerization. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | IONIC LIQUIDS ; ENZYME ; SYSTEM ; STABILITY ; SOLVENTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298555400025 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6639]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Shandong Inst Commerce & Technol, Dept Biotechnol, Jinan 250103, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Yinglong,Zhang Haibo,Zhu Youshuang,et al. The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(69):15647-15655. |
| APA | Zhang Yinglong,Zhang Haibo,Zhu Youshuang,&Huang Feng.(2011).The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(69),15647-15655. |
| MLA | Zhang Yinglong,et al."The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.69(2011):15647-15655. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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