Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography
文献类型：期刊论文
| 作者 | Fa, Yun1 ; Yang, Haiyan1; Ji, Chengshuai2; Cui, He3; Zhu, Xinshu4; Du, Juan5; Gao, Jun1
|
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2013-10-10 |
| 卷号 | 798页码:97-102 |
| 关键词 | Ion chromatography Valve switching Amino acids Carbohydrates Electrochemical detector |
| 英文摘要 | An improved method for the simultaneous determination of 20 amino acids and 7 carbohydrates using one-valve switching after injection, ion chromatography, and integrated pulsed amperometric detection is proposed. The resolution of the amino acids and carbohydrates in the cation trap column was investigated. In addition, parameters including flow liquid type, flow rate, concentration, and valve-switch timing were optimized. The method is time-saving, effective, and accurate for the simultaneous separation of amino acids and carbohydrates, with a mean correlation coefficient of >0.99 and repeatability of 0.5-4.6% for eight replicates. The method was successfully applied in the analysis of amino acids and carbohydrates in aseptic media and in extracellular culture media of three phenotypes of Clostridium thermocellum. (C) 2013 Elsevier B. V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324891500011 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6038]  |
| 专题 | 青岛生物能源与过程研究所_条件保障部公共实验室 |
| 作者单位 | 1.Chinese Acad Sci, Publ Lab Bioenergy & Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.China Univ Petr, Qingdao Econ & Technol Dev Zone, Qingdao 266580, Peoples R China 3.Shandong Entry Exit Inspect & Quarantine Bur, Ctr Tech, Qingdao 266002, Peoples R China 4.Chinese Acad Sci, Metabol Grp, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 5.Qingdao Univ Sci & Technol, Coll Mat Sci & Engn, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fa, Yun,Yang, Haiyan,Ji, Chengshuai,et al. Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography[J]. ANALYTICA CHIMICA ACTA,2013,798:97-102. |
| APA | Fa, Yun.,Yang, Haiyan.,Ji, Chengshuai.,Cui, He.,Zhu, Xinshu.,...&Gao, Jun.(2013).Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography.ANALYTICA CHIMICA ACTA,798,97-102. |
| MLA | Fa, Yun,et al."Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography".ANALYTICA CHIMICA ACTA 798(2013):97-102. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。