Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803
文献类型：期刊论文
| 作者 | Gao, Qianqian1,2; Tan, Xiaoming1; Lu, Xuefeng1 |
| 刊名 | JOURNAL OF BASIC MICROBIOLOGY
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 53期号:10页码:848-855 |
| 关键词 | Fatty acid activation slr1609 Synechocystis sp PCC6803 |
| 英文摘要 | Free fatty acids are typically activated by thioesterification processes and catalyzed by the fatty acyl-CoA synthetase or fatty acyl-ACP synthetase. However, the routes for fatty acid activation in cyanobacteria are not well understood. In this investigation, the slr1609 gene, which encodes the fatty acid activation enzyme, was cloned from Synechocystis sp. PCC6803. This gene was identified by heterologous expression and in vitro enzymatic activity analyses. Different from previous reports stating that free fatty acids are only activated through the fatty acyl-ACP synthetases encoded by these genes in cyanobacteria, this gene was also proven to possess a fatty acyl-CoA synthetase activity, by in vitro enzymatic activity analyses and in vivo complementation experiments. The protein Slr1609 is located in both the cell membrane and the cytosol of Synechocystis sp. PCC6803. The differences in the transcriptional profiles between the wild type and the slr1609 deletion mutant strain were evaluated using microarray analyses. These analyses indicated that 299 differentially expressed genes are involved in fatty acid metabolism, photosynthesis, carbon fixation, stress tolerance and other metabolic processes. Our experiments demonstrate the observed compositional changes in the unsaturated fatty acids from the membrane lipids of the slr1609 deletion mutant when shifted from 30 to 24 degrees C. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | ACYL CARRIER PROTEIN ; SYNTHETASE ; CYANOBACTERIA ; EXPRESSION ; GENES ; PURIFICATION ; ARABIDOPSIS ; PCC-6803 ; MUTANTS ; COLI |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325544500008 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/5993]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Qianqian,Tan, Xiaoming,Lu, Xuefeng. Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803[J]. JOURNAL OF BASIC MICROBIOLOGY,2013,53(10):848-855. |
| APA | Gao, Qianqian,Tan, Xiaoming,&Lu, Xuefeng.(2013).Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803.JOURNAL OF BASIC MICROBIOLOGY,53(10),848-855. |
| MLA | Gao, Qianqian,et al."Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803".JOURNAL OF BASIC MICROBIOLOGY 53.10(2013):848-855. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。