Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria
文献类型：期刊论文
| 作者 | Tan, Xiaoming; Du, Wei; Lu, Xuefeng |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 99期号:5页码:2147-2154 |
| 关键词 | Glucosylglycerol Cyanobacteria Metabolic engineering Gel encapsulation Salt shock Hypoosmotic shock |
| 英文摘要 | Glucosylglycerol (GG) has a range of potential applications in health, pharmacy, and cosmetics due to its physiological, protein-stabilizing, and antioxidative properties. In addition to chemical synthesis and enzymatic catalysis, GG can be produced as a protective osmolyte in salt-stressed bacteria, such as the cyanobacterium Synechocystis sp. PCC 6803. Here, we presented an efficient GG production and secretion by genetically modified and encapsulated Synechocystis cells grown in a semicontinuous manner. We improved the production and secretion of GG in Synechocystis by first disrupting both the ggtC and ggtD genes, which encode the subunits of a GG uptake transporter, as well as the ggpR gene, which encodes a repressor for GG synthesis. Then, we confirmed that the rapid GG release from salt-stressed cells of Synechocystis depended on the ion gradient across the cell membrane. Finally, we proved the feasibility of an agar gel encapsulation method in supporting cell growth and the GG production of Synechocystis under semicontinuous culturing conditions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | STRAIN PCC 6803 ; PHOSPHATE SYNTHASE ; OSMOLYTE SYNTHESIS ; GENE ; GGPS ; BIOSYNTHESIS ; EXPRESSION ; PCC-6803 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350029000010 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6395]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Xiaoming,Du, Wei,Lu, Xuefeng. Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(5):2147-2154. |
| APA | Tan, Xiaoming,Du, Wei,&Lu, Xuefeng.(2015).Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(5),2147-2154. |
| MLA | Tan, Xiaoming,et al."Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.5(2015):2147-2154. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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