Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions
文献类型：期刊论文
| 作者 | Wang, Jian5; Zhu, Jiangfeng4; Bennett, George N.3; San, Ka-Yiu1,2 |
| 刊名 | BIOTECHNOLOGY PROGRESS
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 27期号:5页码:1242-1247 |
| 关键词 | Escherichia coli fermentation sucrose aerobic succinate production |
| 英文摘要 | Two metabolically engineered E. coli strains HL2765k and HL27659k, while capable of producing succinate from glucose with high yields, are not able to grow and produce succinate on sucrose. Consequently, the pUR400 plasmid containing scrK, Y, A, B, and R genes was introduced into HL2765k and HL27659k, respectively. Shake flask culture studies showed that the resulting strains can utilize sucrose; the strain HL2765k pUR400 and HL27659k pUR400 can produce succinate aerobically with a molar yield of 0.78 +/- 0.02 mol/mol and 1.35 +/- 0.13 mol/mol, respectively. On introduction of the plasmid pHL413, which encodes the heterologous pyruvate carboxylase (PYC) from Lactococcus lactis, the molar succinate yield increased to 1.60 +/- 0.01 mol of succinate per mole of sucrose by the HL2765k pUR400 pHL413 strain and to 1.84 +/- 0.10 by the HL27659k pUR400 pHL413 strain. In aerobic batch bioreactor studies, the succinate production rate was faster, and succinate production reached 101.83 mM with a yield of 1.90 when dissolved oxygen (DO) was controlled at 40 +/- 7%. In addition, the results showed that DO had an important effect on succinate production by influencing PYC activity. This work demonstrates the possibility of producing succinate aerobically using sucrose as the carbon source. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 1242-1247, 2011 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 关键词[WOS] | PYRUVATE-CARBOXYLASE ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; ACID PRODUCTION ; MUTANT STRAINS ; OVEREXPRESSION ; PATHWAYS ; PLASMID ; FERMENTATION ; EVOLUTION ; INDUSTRY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296061900006 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6628]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | 1.Rice Univ, Dept Bioengn, Houston, TX 77030 USA 2.Rice Univ, Dept Chem & Biomol Engn, Houston, TX 77251 USA 3.Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77251 USA 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 5.Jilin Univ, Coll Agr & Biol Engn, Changchun 130023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jian,Zhu, Jiangfeng,Bennett, George N.,et al. Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions[J]. BIOTECHNOLOGY PROGRESS,2011,27(5):1242-1247. |
| APA | Wang, Jian,Zhu, Jiangfeng,Bennett, George N.,&San, Ka-Yiu.(2011).Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions.BIOTECHNOLOGY PROGRESS,27(5),1242-1247. |
| MLA | Wang, Jian,et al."Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions".BIOTECHNOLOGY PROGRESS 27.5(2011):1242-1247. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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