RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically
文献类型：期刊论文
| 作者 | Tan, Yang1,2; Liu, Juanjuan1; Chen, Xiaohua1; Zheng, Huajun3; Li, Fuli1
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| 刊名 | MOLECULAR BIOSYSTEMS
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| 出版日期 | 2013 |
| 卷号 | 9期号:11页码:2775-2784 |
| 英文摘要 | Clostridium ljungdahlii DSM 13528 represents a promising platform organism for production of a whole variety of different biofuels and biochemicals from syngas. Although the publication of its genome gave us the first possibility to understand the molecular mechanism for carbon utilization, reports on the profiling of the transcriptome were unavailable. In this study, RNA-seq-based global transcriptome analysis was performed to compare the transcriptomes of C. ljungdahlii grown on CO-CO2 with those grown on fructose. In total, 1852 differentially expressed genes were identified, which included 366 upregulated genes and 1486 downregulated genes under CO-CO2 conditions. These up- and down-regulated genes are predicted to be involved in the Wood-Ljungdahl pathway, CO2 reduction to acetic acid, fructose fermentation, central carbon metabolism and transport, and vitamin B12 synthesis. In addition, 36 small RNAs were identified, 20 of which were novel small RNAs. Quantitative real-time PCR (qRT-PCR) and RT-PCR analysis of the selected functional genes and sRNA genes expression profiles were found to be consistent with the RNA-seq data. The study allowed a deeper understanding of the molecular mechanisms underlying syngas utilization and could help guide the design of rational strategies to increase the efficiency of syngas fixation in the future. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SUBTILIS-TYRS GENE ; 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE ; NONCODING RNAS ; ANTITERMINATION ; GENOME ; IDENTIFICATION ; DEHYDROGENASE ; PURIFICATION ; DISCOVERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325401800019 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/5957]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Yang,Liu, Juanjuan,Chen, Xiaohua,et al. RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically[J]. MOLECULAR BIOSYSTEMS,2013,9(11):2775-2784. |
| APA | Tan, Yang,Liu, Juanjuan,Chen, Xiaohua,Zheng, Huajun,&Li, Fuli.(2013).RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically.MOLECULAR BIOSYSTEMS,9(11),2775-2784. |
| MLA | Tan, Yang,et al."RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically".MOLECULAR BIOSYSTEMS 9.11(2013):2775-2784. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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