Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain
文献类型：期刊论文
| 作者 | Tan, Yang1,2; Liu, Zi-Yong1; Liu, Zhen1 ; Zheng, Hua-Jun3; Li, Fu-Li1
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| 刊名 | MOLECULAR BIOSYSTEMS
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| 出版日期 | 2015 |
| 卷号 | 11期号:5页码:1434-1442 |
| 英文摘要 | The genome of Clostridium acetobutylicum contains the gene encoding CsrA, a carbon storage regulator. We investigated the function of CsrA in C. acetobutylicum by insertionally inactivating the encoding gene, CA_C2209 using the ClosTron. Disruption of csrA obviously decreases the growth of the organism and reduces the yield of acetone, butanol and ethanol (ABEs). Like the csrA in Escherichia coli, RNA-seq and beta-galactosidase analysis revealed that csrA in C. acetobutylicum was closely involved in regulating multiple pathways including flagella assembly, oligopeptide transporting, iron uptake, and central carbon metabolism. It has also been newly demonstrated that csrA in C. acetobutylicum is related to the regulation of pathways involved in the phosphotransferase transporting systems, synthesis of riboflavin, and stage III sporulation. This research represented the first investigation of global regulation by CsrA in the strain belonging to Gram-positive bacteria through transcriptome analysis and provided the important theoretical evidence for improving solvent production by transcriptor engineering in C. acetobutylicum. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | RNA-BINDING PROTEIN ; ENTERICA SEROVAR TYPHIMURIUM ; CAROTOVORA SUBSP CAROTOVORA ; PSEUDOMONAS-AERUGINOSA RSMA ; AIRWAY EPITHELIAL-CELLS ; GLOBAL REGULATORY GENE ; ESCHERICHIA-COLI ; MESSENGER-RNA ; ATCC 824 ; BUTANOL PRODUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353221100022 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6452]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioprocess Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Natl Human Genome Ctr, Shanghai MOST Key Lab Hlth & Dis Gen, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Yang,Liu, Zi-Yong,Liu, Zhen,et al. Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain[J]. MOLECULAR BIOSYSTEMS,2015,11(5):1434-1442. |
| APA | Tan, Yang,Liu, Zi-Yong,Liu, Zhen,Zheng, Hua-Jun,&Li, Fu-Li.(2015).Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain.MOLECULAR BIOSYSTEMS,11(5),1434-1442. |
| MLA | Tan, Yang,et al."Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain".MOLECULAR BIOSYSTEMS 11.5(2015):1434-1442. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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