Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores
文献类型：期刊论文
| 作者 | Liu, Zhen1,2; Qiao, Kai3; Tian, Lei1,2; Zhang, Quan3; Liu, Zi-Yong1,2; Li, Fu-Li1,2,3 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2015-09-09 |
| 卷号 | 6 |
| 关键词 | autolysis sporulation Clostridium acetobutylicum ClosTron autolysin |
| 英文摘要 | Autolysis is a widespread phenomenon in bacteria. In batch fermentation of Clostridium acetobutylicum ATCC 824, there is a spontaneous large-scale autolysis phenomenon with significant decrease of cell density immediately after exponential phase. To unravel the role of autolysis, an autolysin-coding gene, CA_C0554, was disrupted by using ClosTron system to obtain the mutant C. acetobutylicum lyc::int(72). The lower final cell density and faster cell density decrease rate of C. acetobutylicum ATCC 824 than those of C. acetobutylicum lyc::int(72) indicates that CA_C0554 was an important but not the sole autolysin-coding gene responding for the large-scale autolysis. Similar glucose utilization and solvents production but obvious lower cell density of C. acetobutylicum ATCC 824 comparing to C. acetobutylicum lyc::int(72) suggests that lysed C. acetobutylicum ATCC 824 cells were metabolic inactive. On the contrary, the spore density of C. acetobutylicum ATCC 824 is 26.1% higher than that of C. acetobutylicum lyc::int(72) in the final culture broth of batch fermentation. We speculated that spontaneous autolysis of metabolic-inactive cells provided nutrients for the sporulating cells. The present study suggests that one important biological role of spontaneous large-scale autolysis in C. acetobutylicum ATCC 824 batch fermentation is contributing to generation of more spores during sporulation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | BACILLUS-SUBTILIS 168 ; ESCHERICHIA-COLI ; DAIRY PROPIONIBACTERIA ; SOLVENT PRODUCTION ; LYTIC ENZYMES ; GENE ; PEPTIDOGLYCAN ; FERMENTATION ; PURIFICATION ; SPORULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361157900002 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6493]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Sinopec Fushun Res Inst Petr & Petrochem, Fushun, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhen,Qiao, Kai,Tian, Lei,et al. Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores[J]. FRONTIERS IN MICROBIOLOGY,2015,6. |
| APA | Liu, Zhen,Qiao, Kai,Tian, Lei,Zhang, Quan,Liu, Zi-Yong,&Li, Fu-Li.(2015).Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores.FRONTIERS IN MICROBIOLOGY,6. |
| MLA | Liu, Zhen,et al."Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores".FRONTIERS IN MICROBIOLOGY 6(2015). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。