Characterization of an acetoin reductase/2,3-butanediol dehydrogenase from Clostridium ljungdahlii DSM 13528
文献类型：期刊论文
| 作者 | Tan, Yang; Liu, Zi-Yong; Liu, Zhen; Li, Fu-Li |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 79-80页码:1-7 |
| 关键词 | Clostridium ljungdahlii Clostridium acetobutylicum Acetoin reductase 2 Alcohol dehydrogenase 3-Butanediol |
| 英文摘要 | Acetoin reductase catalyzes the formation of 2,3-butanediol from acetoin. In Clostridium ljungdahlii DSM 13528, the gene CLJU_c23220 encoding the putative Zn2+-dependent alcohol dehydrogenase was cloned and expressed in Escherichia coli. The recombinant enzyme, CLAR, can catalyze the conversion of acetoin to 2,3-butanediol with NADPH as the cofactor. Furthermore, the gene CLJU_c23220 was introduced into Clostridium acetobutylicum ATCC 824 and the transformant was conferred the capacity of 2,3-butanediol production. In batch fermentation the transformant produced up to 3.1 g/L of 2,3-butanediol, as well as acetone, butanol and ethanol (ABE, 17.8 g/L) in amounts similar to those produced by the wild type strain. This study provides conclusive evidence at the protein level that CLJU_c23220 is the key gene responsible for the conversion of acetoin to 2,3-butanediol in C. ljurtgdahlii DSM 13528. Moreover, the C. acetobutylicum ATCC 824 was modified via one-step metabolic engineering to produce 2,3-butanediol without influencing the ABE production. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | ENHANCED 2,3-BUTANEDIOL PRODUCTION ; GENE ; ACETOBUTYLICUM ; FERMENTATION ; EXPRESSION ; SEQUENCE ; SERVER ; ACID ; GAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361935100001 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6675]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Yang,Liu, Zi-Yong,Liu, Zhen,et al. Characterization of an acetoin reductase/2,3-butanediol dehydrogenase from Clostridium ljungdahlii DSM 13528[J]. ENZYME AND MICROBIAL TECHNOLOGY,2015,79-80:1-7. |
| APA | Tan, Yang,Liu, Zi-Yong,Liu, Zhen,&Li, Fu-Li.(2015).Characterization of an acetoin reductase/2,3-butanediol dehydrogenase from Clostridium ljungdahlii DSM 13528.ENZYME AND MICROBIAL TECHNOLOGY,79-80,1-7. |
| MLA | Tan, Yang,et al."Characterization of an acetoin reductase/2,3-butanediol dehydrogenase from Clostridium ljungdahlii DSM 13528".ENZYME AND MICROBIAL TECHNOLOGY 79-80(2015):1-7. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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