Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli
文献类型：期刊论文
| 作者 | Chang, Ziwei1,2; Lu, Ming3; Ma, Yunqi1,2; Kwag, Dong-Geon1,2; Kim, Seo-Hyun1,2; Park, Ji-Min1,2; Nam, Bo-Hye4; Kim, Young-Ok4; An, Cheul-Min4; Li, Huayue5 |
| 刊名 | AMINO ACIDS
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 47期号:3页码:579-587 |
| 关键词 | Disulfide bond Escherichia coli Carrier protein Mass spectrometry |
| 英文摘要 | Recombinant expression in Escherichia coli allows the simple, economical, and effective production of bioactive peptides. On the other hand, the production of native peptides, particularly those rich in disulfide bonds, is a major problem. Previous studies have reported that the use of carrier proteins for fusion expression can result in good peptide yields, but few are folded correctly. In this study, two transmembrane small proteins in E. coli, YoaJ and YkgR, which both orientate with their N-termini in cytoplasm and their C-termini in periplasm, were used for fusion expression. The recombinant production of two peptides, asteropsin A (ASPA) and beta-defensin (BD), was induced in the periplasm of E. coli using a selected carrier protein. Both peptides were expressed at high levels, at yields of approximately 5-10 mg/L of culture. Mass spectrometry showed that the resulting peptide had the same molecular weight as their natural forms. After purification, single peaks were observed by reversed phase high-performance liquid chromatography (RP-HPLC), demonstrating the absence of isoforms. Furthermore, cytoplasmically expressed fusion proteins with a carrier at their C-termini did not contain disulfide bonds. This study provides new carrier proteins for fusion expression of disulfide bond-rich peptides in E. coli. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ANTIMICROBIAL PEPTIDES ; BACTERIAL PERIPLASM ; SYSTEM ; THIOREDOXIN ; ANTIBODIES ; ISOMERASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349390700013 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6726]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea 2.Pusan Natl Univ, Chem Inst Funct Mat, Pusan 609735, South Korea 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 4.Natl Fisheries Res & Dev Inst, Aquaculture Ind Dept, Biotechnol Res Div, Pusan 619902, South Korea 5.Pusan Natl Univ, Coll Pharm, Pusan 609735, South Korea |
| 推荐引用方式 GB/T 7714 | Chang, Ziwei,Lu, Ming,Ma, Yunqi,et al. Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli[J]. AMINO ACIDS,2015,47(3):579-587. |
| APA | Chang, Ziwei.,Lu, Ming.,Ma, Yunqi.,Kwag, Dong-Geon.,Kim, Seo-Hyun.,...&Park, Jang-Su.(2015).Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli.AMINO ACIDS,47(3),579-587. |
| MLA | Chang, Ziwei,et al."Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli".AMINO ACIDS 47.3(2015):579-587. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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