热门
Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes
文献类型：期刊论文
| 作者 | Xia, Fan1,2; Zuo, Xiaolei2; Yang, Renqiang3 ; Xiao, Yi2; Kang, Di2; Vallee-Belisle, Alexis2; Gong, Xiong1; Yuen, Jonathan D.1; Hsu, Ben B. Y.1; Heeger, Alan J.1
|
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2010-06-15 |
| 卷号 | 107期号:24页码:10837-10841 |
| 关键词 | biosensor aptamer visual detection thrombin detection cocaine detection |
| 英文摘要 | We have demonstrated a novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid (DNA) sequences, proteins, small molecules, and inorganic ions. This nearly "universal" biosensor approach is based on the observation that, while the conjugated polyelectrolyte specifically inhibits the ability of single-stranded DNA to prevent the aggregation of gold-nanoparticles, no such inhibition is observed with double-stranded or otherwise "folded" DNA structures. Colorimetric assays employing this mechanism for the detection of hybridization are sensitive and convenient-picomolar concentrations of target DNA are readily detected with the naked eye, and the sensor works even when challenged with complex sample matrices such as blood serum. Likewise, by employing the binding-induced folding or association of aptamers we have generalized the approach to the specific and convenient detection of proteins, small molecules, and inorganic ions. Finally, this new biosensor approach is quite straightforward and can be completed in minutes without significant equipment or training overhead. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SEQUENCE-SPECIFIC DETECTION ; OPTICAL-PROPERTIES ; NUCLEIC-ACIDS ; STRANDED-DNA ; SENSOR ; APTAMERS ; COCAINE ; OLIGONUCLEOTIDES ; BIOSENSOR ; AFFINITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278807400014 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6530]  |
| 专题 | 青岛生物能源与过程研究所_先进有机功能材料团队 |
| 作者单位 | 1.Univ Calif Santa Barbara, Ctr Polymers & Organ Solids, Santa Barbara, CA 93106 USA 2.Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Fan,Zuo, Xiaolei,Yang, Renqiang,et al. Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(24):10837-10841. |
| APA | Xia, Fan.,Zuo, Xiaolei.,Yang, Renqiang.,Xiao, Yi.,Kang, Di.,...&Plaxco, Kevin W..(2010).Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,107(24),10837-10841. |
| MLA | Xia, Fan,et al."Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.24(2010):10837-10841. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。