Analysis of Polar Lipids in Chlamydomonas reinhardtii Using Nanoelectrospray Direct Infusion Method and Gas Chromatography and Mass Spectrometric Detection
文献类型：期刊论文
| 作者 | Yang Dawei1,2,3; Fan Xiaolei1 ; Kind, Tobias3; Friehn, Oliver3; Guo Rongbo1
|
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2013-04-15 |
| 卷号 | 71期号:4页码:663-669 |
| 关键词 | polar lipids Chlamydomonas reinhardtii direct infusion method gas chromatography-mass spectrometry free fatty acids |
| 英文摘要 | Recently, microalgae as feedstock for lipid-based biofuel research has evoked great concern around the world. Previous lipid analyses focused on fatty acid esters, triglyceride and total lipid content in algae, but ignored the polar lipids analysis. Here, we present a comprehensive method to cover polar lipids and free fatty acids in Chlamydomonas reinhardtii. Firstly, lipid extraction was performed by methyl-tert-butyl ether which is well suited for shotgun lipidomics method. Afterwards, we developed a fast detection method in polar lipids analysis of C. reinhardtii by using a chip-based nanoelectrospray direct infusion with ion trap mass spectrometry (Nanomate-LTQ). A data-dependent MS/MS method acquired tandem mass spectra in positive and negative mode with a range of 10 minutes infusion time. MS/MS data obtained from Nanomate-LTQ were searched against automated MS/MS database with a precursor window of 0.4 Da and a product ion search window of 0.8 Da. Hit score represents the level of confidence from the Lipidblast library search which can be achieved by free availably NIST MS Search GUI. Therefore, thirty-five lipids were annotated by means of mass precursor search and subsequent in-silico MS/MS mass spectral database (Lipidblast) search, including most polar lipid species which are most common lipid species reported for C. reinhardtii. Among them, twenty classes of diacylglyceryltrimethylhomoserine that are very special lipid species in C. reinhardtii belonging to betaine lipids were annotated with in-house Lipidblast database. In order to validate this Lipidblast match, we performed a manual spectral interpretation of every experimental MS/MS spectrum to ensure authentic ester annotations. Besides, we also applied a gas chromatography tandem mass spectrometric method to detect free fatty acids in C. reinhardtii and found that linolenic acid, linoleic acid and palmitic acid are most abundant free fatty acids in C. reinhardtii. This study provides a potential comprehensive lipidomic method for further lipid analysis in algal research. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | FATTY-ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318258800028 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/5952]  |
| 专题 | 青岛生物能源与过程研究所_生物制氢与沼气团队 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuel, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.UC Davis Genome Ctr Metabol, Davis, CA 95616 USA |
| 推荐引用方式 GB/T 7714 | Yang Dawei,Fan Xiaolei,Kind, Tobias,et al. Analysis of Polar Lipids in Chlamydomonas reinhardtii Using Nanoelectrospray Direct Infusion Method and Gas Chromatography and Mass Spectrometric Detection[J]. ACTA CHIMICA SINICA,2013,71(4):663-669. |
| APA | Yang Dawei,Fan Xiaolei,Kind, Tobias,Friehn, Oliver,&Guo Rongbo.(2013).Analysis of Polar Lipids in Chlamydomonas reinhardtii Using Nanoelectrospray Direct Infusion Method and Gas Chromatography and Mass Spectrometric Detection.ACTA CHIMICA SINICA,71(4),663-669. |
| MLA | Yang Dawei,et al."Analysis of Polar Lipids in Chlamydomonas reinhardtii Using Nanoelectrospray Direct Infusion Method and Gas Chromatography and Mass Spectrometric Detection".ACTA CHIMICA SINICA 71.4(2013):663-669. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。