In order to get experimental data on apple rootstock with iron-efficient genotypes capable of improving scion resistance to iron deficiency, this experiment was conducted on the physiological and molecular characteristics of Fuji/Chistock 1 (F/C) under different iron conditions and compared it to Fuji/M. Baccata (F/B). F/C was less sensitive to iron deficiency than F/B. F/B showed chlorosis after 25 days under iron-deficient conditions, but F/C showed no phenotypic changes, even after 40 days. The shoot and leaf area growth of F/C were respectively 5cm and 1000 mm(2) higher than those of F/B, regardless of the iron-deficient or iron-efficient conditions. The young leaf chlorophyll and active iron of F/C were 5 SPAD and 5 mg kg(-1) higher than those of F/B, either in irondeficient or iron-efficient conditions. The expression of YSL5 and CS1 showed the same pattern. The enhancement expression of iron transport genes may be one explanation for these findings.