Nannochloropsis have attracted sustained interest from algal biodiesel researchers due to their high biomass accumulation rate and high lipid content. There are six recognized species in the Nannochloropsis genus that are phylogenetically divided into Nannochloropsis gaditana, Nannochloropsis salina, Nannochloropsis granulata, Nannochloropsis limnetica, Nannochloropsis oceanica and Nannochloropsis oculata. In this study, the potential of 9 Nannochloropsis species from the 6 genus for biodiesel production was evaluated by determining their growth rate, biomass accumulation, lipid productivity, lipid composition, fatty acid profiles and biodiesel properties. The results showed that the best strain was N. oceanica IMET1, with lipid productivity of 158.76 +/- 13.83 mg L (1) day (1), TAG production of 1.67 +/- 0.20 g/L, favorable fatty acid profiles of C16-C18 (56.62 +/- 1.96%) as well as suitable biodiesel properties of higher cetane number (54.61 +/- 0.25), lower iodine number (104.85 +/- 2.80 gI(2)/100 g) and relative low cloud point (3.45 +/- 0.50 degrees C). N. oceanica IMET1 could be consider as valuable feedstock for microalgal biodiesel production. (C) 2014 Elsevier Ltd. All rights reserved.