Poplar PdC3H17 and PdC3H18 are direct targets of PdMYB3 and PdMYB21, and positively regulate secondary wall formation in Arabidopsis and poplar
文献类型：期刊论文
| 作者 | Chai, Guohua1; Qi, Guang1; Cao, Yingping1; Wang, Zengguang1; Yu, Li1; Tang, Xianfeng1; Yu, Yanchong1; Wang, Dian1; Kong, Yingzhen2; Zhou, Gongke1 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 203期号:2页码:520-534 |
| 关键词 | Arabidopsis cytoplasmic foci PdC3H17 and PdC3H18 poplar xylem development |
| 英文摘要 | Wood biomass is mainly made of secondary cell walls, whose formation is controlled by a multilevel network. The tandem CCCH zinc finger (TZF) proteins involved in plant secondary wall formation are poorly understood. Two TZF genes, PdC3H17 and PdC3H18, were isolated from Populus deltoides and functionally characterized in Escherichia coli, tobacco, Arabidopsis and poplar. PdC3H17 and PdC3H18 are predominantly expressed in cells of developing wood, and the proteins they encode are targeted to cytoplasmic foci. Transcriptional activation assays showed that PdMYB2/3/20/21 individually activated the PdC3H17 and PdC3H18 promoters, but PdMYB3/21 were most significant. Electrophoretic mobility shift assays revealed that PdMYB3/21 bound directly to the PdC3H17/18 promoters. Overexpression of PdC3H17/18 in poplar increased secondary xylem width and secondary wall thickening in stems, whereas dominant repressors of them had the opposite effects on these traits. Similar alteration in secondary wall thickening was observed in their transgenic Arabidopsis plants. qRT-PCR results showed that PdC3H17/18 regulated the expression of cellulose, xylan and lignin biosynthetic genes, and several wood-associated MYB genes. These results demonstrate that PdC3H17 and PdC3H18 are the targets of PdMYB3 and PdMYB21 and are an additional two components in the regulatory network of secondary xylem formation in poplar. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | ZINC-FINGER PROTEIN ; NAC TRANSCRIPTION FACTORS ; WOOD FORMATION ; MODEL SYSTEM ; BIOSYNTHESIS ; MYB46 ; EXPRESSION ; DOMAIN ; GENES ; SND1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337639800018 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6200]  |
| 专题 | 青岛生物能源与过程研究所_植物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resource, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chai, Guohua,Qi, Guang,Cao, Yingping,et al. Poplar PdC3H17 and PdC3H18 are direct targets of PdMYB3 and PdMYB21, and positively regulate secondary wall formation in Arabidopsis and poplar[J]. NEW PHYTOLOGIST,2014,203(2):520-534. |
| APA | Chai, Guohua.,Qi, Guang.,Cao, Yingping.,Wang, Zengguang.,Yu, Li.,...&Zhou, Gongke.(2014).Poplar PdC3H17 and PdC3H18 are direct targets of PdMYB3 and PdMYB21, and positively regulate secondary wall formation in Arabidopsis and poplar.NEW PHYTOLOGIST,203(2),520-534. |
| MLA | Chai, Guohua,et al."Poplar PdC3H17 and PdC3H18 are direct targets of PdMYB3 and PdMYB21, and positively regulate secondary wall formation in Arabidopsis and poplar".NEW PHYTOLOGIST 203.2(2014):520-534. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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