Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation
文献类型：期刊论文
| 作者 | Tang, Xianfeng1; Zhuang, Yamei1,2; Qi, Guang1; Wang, Dian1; Liu, Huanhuan1; Wang, Kairong2; Chai, Guohua1; Zhou, Gongke1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-07-16 |
| 卷号 | 5 |
| 英文摘要 | Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characterized in tree species. Here, we show that PdMYB221, a poplar ortholog of the Arabidopsis R2R3-MYB transcription factor AtMYB4, directly regulates secondary wall biosynthesis during wood formation. PdMYB221 is predominantly expressed in cells of developing wood, and the protein it encodes localizes to the nucleus and acts as a transcriptional repressor. Ectopic expression of PdMYB221 resulted in reduced cell wall thicknesses of fibers and vessels in Arabidopsis inflorescence stems. The amounts of cellulose, xylose, and lignin were decreased and the expression of key genes synthesizing the three components was suppressed in PdMYB221 overexpression plants. Transcriptional activation assays showed that PdMYB221 repressed the promoters of poplar PdCESA7/8, PdGT47C, PdCOMT2 and PdCCR1. Electrophoretic mobility shift assays revealed that PdMYB221 bound directly to the PdCESA8, PdGT47C, and PdCOMT2 promoters. Together, our results suggest that PdMYB221 may be involved in the negative regulation of secondary wall formation through the direct and indirect suppression of the gene expression of secondary wall biosynthesis. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MYB TRANSCRIPTION FACTOR ; FUNCTIONAL-CHARACTERIZATION ; ARABIDOPSIS ; POPULUS ; EXPRESSION ; LIGNIN ; FAMILY ; CELLULOSE ; TOBACCO ; XYLEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358009300001 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6515]  |
| 专题 | 青岛生物能源与过程研究所_植物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Qingdao Agr Univ, Coll Resources & Environm, Qingdao Engn Res Ctr Rural Environm, Qingdao 266109, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Xianfeng,Zhuang, Yamei,Qi, Guang,et al. Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Tang, Xianfeng.,Zhuang, Yamei.,Qi, Guang.,Wang, Dian.,Liu, Huanhuan.,...&Zhou, Gongke.(2015).Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation.SCIENTIFIC REPORTS,5. |
| MLA | Tang, Xianfeng,et al."Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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