Development of a gel monolithic column polydimethylsiloxane microfluidic device for rapid electrophoresis separation
文献类型：期刊论文
| 作者 | Zeng, HL; Li, HF; Wang, X; Lin, JM |
| 刊名 | TALANTA
 |
| 出版日期 | 2006 |
| 卷号 | 69期号:1页码:226-231 |
| 关键词 | PDMS microfluidic device monolithic column chiral separation FITC-labeled amino acid |
| 英文摘要 | A beta-cyclodextrin (beta-CD)-bonded gel monolithic column polydimethylsiloxane (PDMS) microfluidic device was developed in a simple and feasible way. Before preparation of gel monolithic column in PDMS microchannel, PDMS surface was activated by UV light to create silanol groups, which is an active molecule to covalently bond 3-(trimethoxysilyl)-propyl methacrylate (Bind-Silane) and seal microfluidic device. By the way, Bind-Silane is a bifunctional molecule to link polyacrylamide (PAA) gel and inner wall of PDMS microchannel covalently. Allyl-beta-CD was used not only as a multifunctional crosslinker in PAA gel to control the size of the pores, but also as a chiral selector for the enantioseparation. The stability, transferring heat and optical characteristic of the microfluidic device were examined. The separation capability of the gel monolithic column was confirmed by the successful separation of fluorescein isothiocyanate (FITC)-tabeled arginine (Arg), glutamine acid (Glu), tryptophan (Try), cysteine (Cysteine) and phenylalanine (Phe) in the PDMS microfluidic device less than 100 s at 36 mm effective separation length. A maximum of 2.06 x 10(5) theoretical plates was obtained by the potential strength of 490 V/cm. A pair of FITC-labeled dansyl-D,L-threonine (Dns-Thr) was separated absolutely. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000235510000035 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22863]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zeng, HL,Li, HF,Wang, X,et al. Development of a gel monolithic column polydimethylsiloxane microfluidic device for rapid electrophoresis separation[J]. TALANTA,2006,69(1):226-231. |
| APA | Zeng, HL,Li, HF,Wang, X,&Lin, JM.(2006).Development of a gel monolithic column polydimethylsiloxane microfluidic device for rapid electrophoresis separation.TALANTA,69(1),226-231. |
| MLA | Zeng, HL,et al."Development of a gel monolithic column polydimethylsiloxane microfluidic device for rapid electrophoresis separation".TALANTA 69.1(2006):226-231. |
入库方式： OAI收割
来源：生态环境研究中心
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