Measurement of estrogenic activity in sediments from Haihe and Dagu River, China
文献类型：期刊论文
| 作者 | Song, Maoyong; Xu, Yan; Jiang, Qinting; Lam, Paul K. S.; O'Toole, Desmond K.; Giesy, John P.; Jiang, Guibin |
| 刊名 | ENVIRONMENT INTERNATIONAL
 |
| 出版日期 | 2006 |
| 卷号 | 32期号:5页码:676-681 |
| 关键词 | estrogenic activity sediments E2-EQs Haihe and Dagu Rivers in vitro bioassay MCF-7 cell line |
| 英文摘要 | Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil colunm into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17 beta-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g(-1) dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 > Fraction2 > Fraction1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17 beta-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 > E1 > NP > OP > BPA. (c) 2006 Elsevier Ltd. All rights reserved. |
| WOS记录号 | WOS:000238521000012 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22890]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Song, Maoyong,Xu, Yan,Jiang, Qinting,et al. Measurement of estrogenic activity in sediments from Haihe and Dagu River, China[J]. ENVIRONMENT INTERNATIONAL,2006,32(5):676-681. |
| APA | Song, Maoyong.,Xu, Yan.,Jiang, Qinting.,Lam, Paul K. S..,O'Toole, Desmond K..,...&Jiang, Guibin.(2006).Measurement of estrogenic activity in sediments from Haihe and Dagu River, China.ENVIRONMENT INTERNATIONAL,32(5),676-681. |
| MLA | Song, Maoyong,et al."Measurement of estrogenic activity in sediments from Haihe and Dagu River, China".ENVIRONMENT INTERNATIONAL 32.5(2006):676-681. |
入库方式： OAI收割
来源：生态环境研究中心
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