Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes
文献类型：期刊论文
| 作者 | Hu, Ligang; Jiang, Guibin; Xu, Songyun; Pan, Chensong; Zou, Hanfa |
| 刊名 | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
 |
| 出版日期 | 2006 |
| 卷号 | 17期号:11页码:1616-1619 |
| 英文摘要 | A matrix assisted laser desorption/ionization time-of-flight mass spectrometry platform for quantitatively monitoring enzyme activity and screening enzyme inhibitors has been demonstrated. The described method employs a new matrix of oxidized carbon nanotubes. Compared with the traditional fluorescence approach, this label-free method has the advantage of directly identifying the substrates and products in enzymatic reactions. Moreover, the method could be conveniently carried out with any commercial mass spectrometer without modification. We quantitatively monitored the acetylcholinesterase activity and screened acetylcholinesterase inhibitors with a detection rate of about 3.3 s per sample. |
| WOS记录号 | WOS:000242242900017 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22898]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Hu, Ligang,Jiang, Guibin,Xu, Songyun,et al. Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2006,17(11):1616-1619. |
| APA | Hu, Ligang,Jiang, Guibin,Xu, Songyun,Pan, Chensong,&Zou, Hanfa.(2006).Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,17(11),1616-1619. |
| MLA | Hu, Ligang,et al."Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 17.11(2006):1616-1619. |
入库方式： OAI收割
来源：生态环境研究中心
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