Chiral separation of dansyl amino acids by PDMS microchip gel monolithic column electrochromatography with gamma-cyclodextrin bonded in polyacrylamide
文献类型：期刊论文
| 作者 | Zeng, HL; Li, HF; Lin, JM |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2005 |
| 卷号 | 551期号:2015-01-02页码:1-8 |
| 关键词 | PDMS microfluidic device monolithic column electrochromatography gamma-CD chiral separation FITC-labeled Dns-amino acid |
| 英文摘要 | A polydimethylsiloxane (PDMS) microchip polyacrylamide (PAA) gel monolithic column for electrochromatography was prepared. A chiral recognizable molecule, gamma-cyclodextrin (gamma-CD) was immobilized firmly in the PAA gel in the microchannel through chemical bond. The PDMS microchannel was pretreated with 3-(trimethoxysilyl)-propyl methacrylate (Bind-Silane) before bonding PAA monolithic column, which was an anchor molecule to connect the PAA gel with PDMS inner wall in microchannel. The experiments demonstrated gamma-CD was a perfect chiral selector for fluorescein isoihiocyanate (FITC) labeled dansyl amino acids (Dns-AAs), which showed well capability for chiral recognization in monolithic column electrochromatography. The mixture of two pairs of FITC-labeled Dns-AAs was separated absolutely in this new-style PDMS-based microfluidic device monolithic column within 100 s in 36 mm effective separation length, and the resolutions of the two pairs of optical isomers could reach 2.53 and 1.12, respectively. The condition of preparation PAA gel monolithic column for chiral separation was optimized by adjusting the concentration of allyl-gamma-CD. The stability, transferring heat and optical characteristic of PDMS-based microfluidic device monolithic column were examined, respectively. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000232505200001 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23151]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zeng, HL,Li, HF,Lin, JM. Chiral separation of dansyl amino acids by PDMS microchip gel monolithic column electrochromatography with gamma-cyclodextrin bonded in polyacrylamide[J]. ANALYTICA CHIMICA ACTA,2005,551(2015-01-02):1-8. |
| APA | Zeng, HL,Li, HF,&Lin, JM.(2005).Chiral separation of dansyl amino acids by PDMS microchip gel monolithic column electrochromatography with gamma-cyclodextrin bonded in polyacrylamide.ANALYTICA CHIMICA ACTA,551(2015-01-02),1-8. |
| MLA | Zeng, HL,et al."Chiral separation of dansyl amino acids by PDMS microchip gel monolithic column electrochromatography with gamma-cyclodextrin bonded in polyacrylamide".ANALYTICA CHIMICA ACTA 551.2015-01-02(2005):1-8. |
入库方式： OAI收割
来源：生态环境研究中心
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