Determination of amino acids in hydrolysate of corn powder and fish powder by high performance anion exchange chromatography with integrated pulsed amperometric detection
文献类型：期刊论文
| 作者 | Cai, YQ; Liu, JS; Mou, SF |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 33期号:4页码:475-478 |
| 关键词 | amino acids high performance anion exchange chromatography integrated pulsed amperometric detection |
| 英文摘要 | A method for the determination of 17 amino acids in hydrolysate of corn powder and fish powder by high performance anion exchange chromatography with integrated pulsed amperometric detection was established. The chromatographic separation of 17 amino acids was carried out on an Dionex Amino Pac PA10 anion exchange column using a ternary gradient elution. The eluent consists of deionized water, 250 mmol/L sodium hydroxide and 1. 0 mol/L sodium acetate, and its flow rate was set at 0. 25 mL/min. Under the optimized conditions, 17 amino acids were separated and detected effectively. RSDs of the retention time and peak area for the five determinations of 17 amino acids in hydrolysate of fish powder were no more than 2. 8% and 4. 7% respectively. The linearities for 17 analytes were two or three orders of magnitude. Detection limits of 0. 21 ∼ 3. 4 pmol for 17 amino acids were achieved under the optimized conditions. The accuracy of the proposed method was tested by recovery measurements on spiked samples and good recovery results were obtained. |
| 学科主题 | Chemistry |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000228870200010 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23160]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Cai, YQ,Liu, JS,Mou, SF. Determination of amino acids in hydrolysate of corn powder and fish powder by high performance anion exchange chromatography with integrated pulsed amperometric detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2005,33(4):475-478. |
| APA | Cai, YQ,Liu, JS,&Mou, SF.(2005).Determination of amino acids in hydrolysate of corn powder and fish powder by high performance anion exchange chromatography with integrated pulsed amperometric detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,33(4),475-478. |
| MLA | Cai, YQ,et al."Determination of amino acids in hydrolysate of corn powder and fish powder by high performance anion exchange chromatography with integrated pulsed amperometric detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 33.4(2005):475-478. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。