Determination of several estrogens in serum by high-performance liquid chromatography with ultraviolet detection
文献类型：期刊论文
| 作者 | Cai, YQ; Wang, L; Liang, LN; Liu, JS; Shi, YL; Mou, SF; Jiang, GB |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 33期号:7页码:913-916 |
| 关键词 | estrogen serum high-performance liquid chromatography ultraviolet detection |
| 英文摘要 | A simple and direct method for determination of four estrogens beta-estradiol, estriol, estrone and 17 alpha-ethynylestradiol in serum sample by high-performance liquid chromatography coupled with ultraviolet detection was developed. The four target analytes were easily and completely separated on a polar-enhanced C-16 silica stationary phase packed reversed-phase column at a flow-rate of 1 mL/min by isocratic elution with mobile phase of acetonitrile/water (40:60, V/V) in about 26 min. The column temperature was kept at 40 degrees C. The separated four analytes were detected directly by UV detector at 200 nm wavelength. Under the optimized conditions, when the injection volume was 20 mu L, the detection limits (signal-to-noise ratio equal to 3) for beta-estradiol, estriol, estrone and 17 alpha-ethynylestradiol were 0.024, 0.015, 0.012 and 0.016 mg/L, respectively. The calibration graphs for the four analytes were linear over two to three orders of magnitude with correlation coefficients greater than 0.998. The analytical method had been applied to the determination of beta-estradiol, estriol, estrone and 17 alpha-ethynylestradiol in real serum samples. The accuracy of the proposed method was tested by recovery measurements on spiked samples and good recovery results (95.2% similar to 103.7%) were obtained. |
| 学科主题 | Chemistry |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000230998500004 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23166]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Cai, YQ,Wang, L,Liang, LN,et al. Determination of several estrogens in serum by high-performance liquid chromatography with ultraviolet detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2005,33(7):913-916. |
| APA | Cai, YQ.,Wang, L.,Liang, LN.,Liu, JS.,Shi, YL.,...&Jiang, GB.(2005).Determination of several estrogens in serum by high-performance liquid chromatography with ultraviolet detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,33(7),913-916. |
| MLA | Cai, YQ,et al."Determination of several estrogens in serum by high-performance liquid chromatography with ultraviolet detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 33.7(2005):913-916. |
入库方式： OAI收割
来源：生态环境研究中心
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