中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Development and validation of an enzyme-linked immunosorbent assay for vitellogenin in Chinese loach (Misgurnus angaillicaudatus)

文献类型:期刊论文

作者Shao, J; Shi, GQ; Song, MY; Jiang, GB
刊名ENVIRONMENT INTERNATIONAL
出版日期2005
卷号31期号:5页码:763-770
关键词enzyme-linked immonosorbent assay vitellogenin Chinese loach Misgurnus angaillicaudatus
英文摘要A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantification of vitellogenin (Vtg) in the plasma of Chinese loach (Misgurnus angaillicaudatus). Vtg was isolated by anion exchange membrane chromatography from the plasma of 17 beta-estradiol (E-2)-treated loach. Polyclonal antibody against Vtg was raised in rabbits, and the specificity of the antibody was assessed by Western blotting analysis. No cross-reactivity was observed with plasma from male loach. Plasma samples from vitellogenic females and E-2-treated mates were diluted parallel with the purified Vtg standard curve in the ELISA. The detection limit of the assay was 5.7 ng ml(-1) and a working range of the standard curve was between 15.6 and 1000 ng ml(-1), The intra- and inter-assay coefficients of variations were 6.9% and 10.4%, respectively. The recovery was 104.4%. The assay was applied for measuring vitellogenin concentration in the plasma from 24 wild Chinese loach from Lianyungang, Jiangsu Province, in October 2003. It was found that, in addition to females (plasma Vtg concentration ranging from 518.4 mu g ml(-1) to 1922.3 mu g ml(-1)), male loach showed low plasma Vtg-from undetectable (two fish) to 2.9 +/- 0.7 mu g ml(-1) (mean +/- S.D.). The results indicate that this method provides a valuable tool for the study of estrogenic effect in Chinese loach. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题Environmental Sciences & Ecology
研究领域[WOS]Environmental Sciences & Ecology
WOS记录号WOS:000229724400016
源URL[http://ir.rcees.ac.cn/handle/311016/23169]  
专题生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式
GB/T 7714
Shao, J,Shi, GQ,Song, MY,et al. Development and validation of an enzyme-linked immunosorbent assay for vitellogenin in Chinese loach (Misgurnus angaillicaudatus)[J]. ENVIRONMENT INTERNATIONAL,2005,31(5):763-770.
APA Shao, J,Shi, GQ,Song, MY,&Jiang, GB.(2005).Development and validation of an enzyme-linked immunosorbent assay for vitellogenin in Chinese loach (Misgurnus angaillicaudatus).ENVIRONMENT INTERNATIONAL,31(5),763-770.
MLA Shao, J,et al."Development and validation of an enzyme-linked immunosorbent assay for vitellogenin in Chinese loach (Misgurnus angaillicaudatus)".ENVIRONMENT INTERNATIONAL 31.5(2005):763-770.

入库方式: OAI收割

来源:生态环境研究中心

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。