Separation and determination of norepinephrine, epinephrine and isoprinaline enantiomers by capillary electrophoresis in pharmaceutical formulation and human serum
文献类型：期刊论文
| 作者 | Wei, SL; Song, GQ; Lin, JM |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2005 |
| 卷号 | 1098期号:2015-01-02页码:166-171 |
| 关键词 | capillary electrophoresis cyclodextrin enantiomer separation norepinephrine (NE) epinephrine (EP) isoprinaline (IP) |
| 英文摘要 | A capillary electrophoresis method with ultraviolet (UV) detection was developed and optimized for the enantiomer separation of norepinephrine (NE), epinephrine (EP) and isoprenaline (IP) using dual cyclodextrins (CDs) of 2-hydroxypropyl-beta-CD (HP-beta-CD) and heptakis (2,6-di-o-methyl)beta-CD (DM-beta-CD) as chiral selectors. Optimal separation was obtained using a running buffer of 50 mM phosphate containing 30 mM HP-beta-CD and 5 mM DM-beta-CD at pH 2.90 and a field strength of 20 kV in 45 cm x 75 mu m (40cm effective length) uncoated capillary. The UV absorbance detection was set at 205 run. A 0.1% (w/w) polyethylene glycol or 0.1% (v/v) acetonitrile was used to enhance the detection sensitivity. There was a wide and excellent linear calibration graph for each enantiomer in the range 1.0 X 10(-3) to 1.0 x 10(-6) M and the detection limit (S/N = 3) was found from 8.5 x 10(-7) to 9.5 x 10(-1) M. The method has been applied for the determination of isoprenaline in isoprenaline hydrochloride aerosol and to the analysis of serum samples. The recoveries of NE and EP in serum and IP in drug were ranged from 90 to 110%, The relative standard deviations of all the analyte peaks were less than 2.8% for migration time and less than 4.8% for peak area. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000233954200022 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23224]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wei, SL,Song, GQ,Lin, JM. Separation and determination of norepinephrine, epinephrine and isoprinaline enantiomers by capillary electrophoresis in pharmaceutical formulation and human serum[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1098(2015-01-02):166-171. |
| APA | Wei, SL,Song, GQ,&Lin, JM.(2005).Separation and determination of norepinephrine, epinephrine and isoprinaline enantiomers by capillary electrophoresis in pharmaceutical formulation and human serum.JOURNAL OF CHROMATOGRAPHY A,1098(2015-01-02),166-171. |
| MLA | Wei, SL,et al."Separation and determination of norepinephrine, epinephrine and isoprinaline enantiomers by capillary electrophoresis in pharmaceutical formulation and human serum".JOURNAL OF CHROMATOGRAPHY A 1098.2015-01-02(2005):166-171. |
入库方式： OAI收割
来源：生态环境研究中心
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