A rapid two-step chromatographic method for the quantitative determination of vitellogenin in fish plasma
文献类型：期刊论文
| 作者 | Shao, J; Shi, GQ; Liu, JF; Jiang, GB |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2004 |
| 卷号 | 378期号:3页码:615-620 |
| 关键词 | Vtg HPSEC membrane purification loach sea catfish |
| 英文摘要 | By combining anion-exchange membrane purification with high-performance size-exclusion chromatography (HPSEC) analysis, a two-step chromatographic method was developed for the determination of vitellogenin (Vtg) in fish plasma. Most plasma protein interferences can be removed during anion-exchange membrane purification process. Vtg is eluted from the size-exclusion chromatography column with a retention time of about 9 min and is characterized based on the native molecular weight, with a limit of quantification of 20 mug Vtg mL(-1) plasma. The spiked recovery and interassay variability were better than 80% and 4.8%. This method was successfully applied to analyze the plasma Vtg levels of loach (Misgurnus angaillicaudatus) and sea catfish (Enchelyopus elongatus). In addition to all the female fish, Vtg is detected in 75% of male loaches and 100% of male sea catfish. The result indicates that some chemicals or unknown factors with estrogenic activity have induced male fish to produce Vtg. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000188749000010 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23421]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Shao, J,Shi, GQ,Liu, JF,et al. A rapid two-step chromatographic method for the quantitative determination of vitellogenin in fish plasma[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2004,378(3):615-620. |
| APA | Shao, J,Shi, GQ,Liu, JF,&Jiang, GB.(2004).A rapid two-step chromatographic method for the quantitative determination of vitellogenin in fish plasma.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,378(3),615-620. |
| MLA | Shao, J,et al."A rapid two-step chromatographic method for the quantitative determination of vitellogenin in fish plasma".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 378.3(2004):615-620. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。