Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip
文献类型：期刊论文
| 作者 | Su, RG; Lin, JM; Uchiyama, K; Yamada, M |
| 刊名 | TALANTA
 |
| 出版日期 | 2004 |
| 卷号 | 64期号:4页码:1024-1029 |
| 关键词 | microchip electrophoresis chemiluminescence detection flow-type detection cell double-T injection |
| 英文摘要 | A glass electrophoresis microchip integrated a flow-type chemluminescence (CL) detection cell has been developed and evaluated. The chip pattern is a double-T-type electrophoretic sample injection and separation combining with a Y-type chemiluminecent detector. The double-T geometry allows for high-efficiency sample injection and geometric definition of sample plug size. The branch of Y was used as CL reagent channel, and the CL reagent was delivered by a tab-made micropump. Bis[(2,4,6-trichlorophenyl)]oxalate-H2O2 CL system was employed to detect dansyl amino acids. On this microchip, dansyl-phenylalanine and -sarcosine were successfully separated by electrophoresis and detected within 250 s. The detection limits (S/N = 3) of dansyl-phenylalanine and -sarcosine could reach to 2.8 and 3.2 muM, respectively, due to the vigorous dilution of sample with CL reagent and timely removal of the waste solution from reaction area. (C) 2004 Elsevier B.V. All rights reserved. |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000224772600032 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23454]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Su, RG,Lin, JM,Uchiyama, K,et al. Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip[J]. TALANTA,2004,64(4):1024-1029. |
| APA | Su, RG,Lin, JM,Uchiyama, K,&Yamada, M.(2004).Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip.TALANTA,64(4),1024-1029. |
| MLA | Su, RG,et al."Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip".TALANTA 64.4(2004):1024-1029. |
入库方式： OAI收割
来源：生态环境研究中心
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