Chiral separation with ligand-exchange micellar electrokinetic chromatography using a D-penicillamine-copper(II) ternary complex as chiral selector
文献类型：期刊论文
| 作者 | Zheng, ZX; Lin, JM; Qu, F; Hobo, T |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:24页码:4221-4226 |
| 关键词 | chiral separation ligand-exchange micellar electrokinetic chromatography D-penicillamine |
| 英文摘要 | D-Penicillamine is demonstrated for the first time as a chiral ligand for the enantioseparation of dansyl amino acids based on ligand-exchange micellar electrokinetic chromatography (LE-MEKC). Copper(II) was used as the central ion in the ternary complex. The effect of surfactant on the resolution was significant. A concentration of 20 mm sodium dodecyl sulfate (SDS) was shown to be necessary for the separation. Other important parameters, such as the concentration ratio of D-penicillamine (D-PEN) to Cu2+, the kind of metal central ion, the type and pH value of buffer, were also investigated. N-Acetyl-D-penicillamine and L-valine (Val), with similar structure to D-penicillamine, were applied as their copper(II) complexes as chiral selector and the chiral recognition mechanism is briefly discussed. Under optimum experimental conditions, i.e., 20 mm NH4OAc, pH 6.5, a 2:1 concentration ratio Of D-penicillamine to Cu(II), 4 mm CuSO4 and 8 MM D-penicillamine, the chiral separation of eight pairs of different dansyl amino acid enantiomers was accomplished with resolution ranging from 1.1 to 5.9. When L-PEN was used instead Of D-PEN, reversal of the migration order was observed. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000187897300008 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23670]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zheng, ZX,Lin, JM,Qu, F,et al. Chiral separation with ligand-exchange micellar electrokinetic chromatography using a D-penicillamine-copper(II) ternary complex as chiral selector[J]. ELECTROPHORESIS,2003,24(24):4221-4226. |
| APA | Zheng, ZX,Lin, JM,Qu, F,&Hobo, T.(2003).Chiral separation with ligand-exchange micellar electrokinetic chromatography using a D-penicillamine-copper(II) ternary complex as chiral selector.ELECTROPHORESIS,24(24),4221-4226. |
| MLA | Zheng, ZX,et al."Chiral separation with ligand-exchange micellar electrokinetic chromatography using a D-penicillamine-copper(II) ternary complex as chiral selector".ELECTROPHORESIS 24.24(2003):4221-4226. |
入库方式： OAI收割
来源：生态环境研究中心
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