Determination of cathepsin D activity in MCF-7 cells by capillary zone electrophoresis with on-column sample stacking
文献类型：期刊论文
| 作者 | Fu, S; Chu, SG; Qin, ZF; Xu, XB |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2003 |
| 卷号 | 58期号:1页码:73-78 |
| 关键词 | capillary electrophoresis cathepsin D MCF-7 |
| 英文摘要 | A rapid and simple capillary zone electrophoretic (CZE) method has been developed for analysis of cathepsin D activity in MCF-7 cells. Enzyme active suspension was separated and incubated with hemoglobin as a substrate. Specific peptides were then separated and analyzed by capillary zone electrophoresis with on-column sample stacking. The CZE conditions, including the organic solvents used, their concentrations, and pH and separation voltage, were optimized. The results showed that dilution of the sample with acetonitrile was compensated by using large-volume injection in the stacking procedure. The limit of determination of the method was dramatically improved. This relatively rapid and simple means of determination of cathepsin D activity was successfully used to assay the expression of cathepsin D in cultured MCF-7 cells, to evaluate the estrogenic activity of hormone-disrupting chemicals present in the environment. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000184494100011 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23674]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Fu, S,Chu, SG,Qin, ZF,et al. Determination of cathepsin D activity in MCF-7 cells by capillary zone electrophoresis with on-column sample stacking[J]. CHROMATOGRAPHIA,2003,58(1):73-78. |
| APA | Fu, S,Chu, SG,Qin, ZF,&Xu, XB.(2003).Determination of cathepsin D activity in MCF-7 cells by capillary zone electrophoresis with on-column sample stacking.CHROMATOGRAPHIA,58(1),73-78. |
| MLA | Fu, S,et al."Determination of cathepsin D activity in MCF-7 cells by capillary zone electrophoresis with on-column sample stacking".CHROMATOGRAPHIA 58.1(2003):73-78. |
入库方式： OAI收割
来源：生态环境研究中心
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