Development and optimization of a method for the analysis of low-molecular-mass organic acids in plants by capillary electrophoresis with indirect UV detection
文献类型：期刊论文
| 作者 | Wang, M; Qu, F; Shan, XQ; Lin, JM |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2003 |
| 卷号 | 989期号:2页码:285-292 |
| 关键词 | plant materials Var splendens organic acids |
| 英文摘要 | Capillary zone electrophoresis was developed to analyze low-molecular-mass organic acids including oxalic, tartaric, formic, malic, citric, succinic, glutaric, acetic and lactic acid. The influences of some crucial parameters such as buffer concentration, pH value, surfactant concentration and separation temperature, on electrophoretic separation were investigated. Under the conditions of 15 mM phthalate containing 0.6 mM tetradecyltrimethylammonium bromide as the run buffer (pH 5.6); separation voltage -15 W (-263.2 V/cm) and temperature 25 degreesC, a satisfactory separation of nine organic acids was accomplished within 7 min. The detection limits (S/N=3) ranged from 0.008 to 0.08 mug/ml and the quantification limits ranged from 0.01 to 0.1 mug/ml for electrokinetic injection. The method was successfully applied to analyze organic acids in different parts of Var splendens (an edible vegetable in China). The recoveries of organic acids in real samples ranged from 88 to 121%. (C) 2003 Elsevier Science B.V. All rights reserved. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000181377200011 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23678]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, M,Qu, F,Shan, XQ,et al. Development and optimization of a method for the analysis of low-molecular-mass organic acids in plants by capillary electrophoresis with indirect UV detection[J]. JOURNAL OF CHROMATOGRAPHY A,2003,989(2):285-292. |
| APA | Wang, M,Qu, F,Shan, XQ,&Lin, JM.(2003).Development and optimization of a method for the analysis of low-molecular-mass organic acids in plants by capillary electrophoresis with indirect UV detection.JOURNAL OF CHROMATOGRAPHY A,989(2),285-292. |
| MLA | Wang, M,et al."Development and optimization of a method for the analysis of low-molecular-mass organic acids in plants by capillary electrophoresis with indirect UV detection".JOURNAL OF CHROMATOGRAPHY A 989.2(2003):285-292. |
入库方式： OAI收割
来源：生态环境研究中心
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