Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma
文献类型：期刊论文
| 作者 | Shi, GQ; Shao, J; Jiang, GB; Wang, QX; Lu, YQ; Liu, JF; Liu, JM |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2003 |
| 卷号 | 785期号:2页码:361-368 |
| 关键词 | vitellogenin |
| 英文摘要 | A membrane chromatographic method was developed for the rapid purification of vitellogenin (Vtg) from the plasma of 17beta-estradiol induced loach (Misgurnus angaillicaud atus) and carp (Cyprinus carpio). The time required for the proposed procedure is less then 10 min at a flow-rate of 5 ml/min of the mobile phase, and 0.5 ml of fish plasma could be separated in one cycle. Multistep gradient elution was more suitable for the separation than linear gradient elution. Under optimized conditions, a single Vtg peak can be obtained and its identity was confirmed by SDS-PAGE and gel-permeation chromatography assessment. This method is rapid and easy to operate compared to conventional HPLC and FPLC columns for Vtg separation. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000180789100019 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23694]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Shi, GQ,Shao, J,Jiang, GB,et al. Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2003,785(2):361-368. |
| APA | Shi, GQ.,Shao, J.,Jiang, GB.,Wang, QX.,Lu, YQ.,...&Liu, JM.(2003).Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,785(2),361-368. |
| MLA | Shi, GQ,et al."Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 785.2(2003):361-368. |
入库方式： OAI收割
来源：生态环境研究中心
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