Displacement chromatography of isomers and therapeutic compounds
文献类型：期刊论文
| 作者 | Qi, Y; Huang, JX |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2002 |
| 卷号 | 959期号:2015-01-02页码:85-93 |
| 关键词 | displacement chromatography preparative chromatography epirubicin doxorubicin |
| 英文摘要 | Displacement chromatography was successfully used to separate a binary isomer mixture, epirubicin and doxorubicin, on Kromasil KR100-10 C-18 250X4.6 mm I.D. (10 mum) column. Displacement parameters such as the types and the concentrations of displacer, the composition and the flow rate of the mobile phase were critically examined in this study. The displacer employed was 30 mg/ml benzethonium chloride. Loading of feed at lower initial organic level of mobile phase coupled with displacement at higher organic level was found to give efficient separation. A 30-mg amount of binary isomer mixture was separated on an analytical column. The purification of epirubicin from the closely related impurities present in raw product solution by displacement chromatography was also investigated. The purity of epirubicin required was greater than 99% with a recovery of 60%. The results have indicated that this process made good use of the high feed load, low solvent costs, and high resolution characteristics of displacement chromatography and offered the chromatographic engineer a powerful tool for the preparative purification of therapeutic compounds. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000176608400008 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23874]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Qi, Y,Huang, JX. Displacement chromatography of isomers and therapeutic compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2002,959(2015-01-02):85-93. |
| APA | Qi, Y,&Huang, JX.(2002).Displacement chromatography of isomers and therapeutic compounds.JOURNAL OF CHROMATOGRAPHY A,959(2015-01-02),85-93. |
| MLA | Qi, Y,et al."Displacement chromatography of isomers and therapeutic compounds".JOURNAL OF CHROMATOGRAPHY A 959.2015-01-02(2002):85-93. |
入库方式： OAI收割
来源：生态环境研究中心
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