Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins
文献类型：期刊论文
| 作者 | Qi, Y; Yan, Z; Huang, JX |
| 刊名 | JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
 |
| 出版日期 | 2001 |
| 卷号 | 49期号:2015-01-03页码:263-273 |
| 关键词 | immunoglobulin G albumin coupled-column high-performance liquid chromatography separation and purification |
| 英文摘要 | A high-performance liquid-chromatographic method based on coupled DEAE anion-exchange and Protein G affinity columns has been developed for the simultaneous separation and purification of immunoglobulin G and albumin from mouse serum. The diluted mouse serum was injected directly into this system, and the proteins were elated separately from the DEAE and Protein G columns, coupled in series, by the column-switching technique. The advantages of this method are that IgG and albumin can be separated and purified simultaneously, the expensive affinity column is protected from contamination by the impurities in the mouse serum, and it is fast, selective, robust, and reproducible. (C) 2001 Elsevier Science B.V. All rights reserved. |
| WOS记录号 | WOS:000172468700016 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23998]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Qi, Y,Yan, Z,Huang, JX. Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,2001,49(2015-01-03):263-273. |
| APA | Qi, Y,Yan, Z,&Huang, JX.(2001).Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins.JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,49(2015-01-03),263-273. |
| MLA | Qi, Y,et al."Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins".JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 49.2015-01-03(2001):263-273. |
入库方式： OAI收割
来源：生态环境研究中心
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