Spectrophotometric flow injection determination of total reducing sugars in tobacco based on oxidation by ferricyanide and formation of Prussian blue
文献类型：期刊论文
| 作者 | Liu, JF; Jiang, GB |
| 刊名 | ANALYTICAL LETTERS
 |
| 出版日期 | 2001 |
| 卷号 | 34期号:11页码:1923-1934 |
| 关键词 | flow injection spectrophotometry Prussian blue on-line hydrolysis total reducing sugars tobacco |
| 英文摘要 | Using the Prussian blue (ferric ferrocyanide) color reaction, a simple and sensitive flow injection spectrophotometric method for the determination of total reducing sugars in tobacco is proposed. At a temperature of 110 degreesC, sucrose was on-line hydrolyzed to reducing sugars quantitatively in 0.5 mol l(-1) HCl. The reducing sugars were oxidized by alkaline potassium ferricyanide solution with equal reaction rates for glucose and fructose. The produced ferrocyanide reacted with ferric nitrate to form Prussian blue, which was detected spectrophotometrically at 690 nm for evaluation of the total reducing sugars. Using a merging zone manifold and alkaline citrate solution as carrier, the precipitation of Prussian blue oil the system tube was effectively prevented. The detection limits and linear ranges were 0.1 mg l(-1) and 0.5-15 mg l(-1) for glucose, and 0.4 mg l(-1) and 0.5-20 mg l(-1) for fructose, respectively. The sampling rate was 40 per hour. The proposed method was applied to determine total reducing sugars in tobacco. |
| WOS记录号 | WOS:000171342300010 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/24038]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, JF,Jiang, GB. Spectrophotometric flow injection determination of total reducing sugars in tobacco based on oxidation by ferricyanide and formation of Prussian blue[J]. ANALYTICAL LETTERS,2001,34(11):1923-1934. |
| APA | Liu, JF,&Jiang, GB.(2001).Spectrophotometric flow injection determination of total reducing sugars in tobacco based on oxidation by ferricyanide and formation of Prussian blue.ANALYTICAL LETTERS,34(11),1923-1934. |
| MLA | Liu, JF,et al."Spectrophotometric flow injection determination of total reducing sugars in tobacco based on oxidation by ferricyanide and formation of Prussian blue".ANALYTICAL LETTERS 34.11(2001):1923-1934. |
入库方式： OAI收割
来源：生态环境研究中心
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