Regeneration of high performance ion exchange chromatographic column in protein separations and purifications
文献类型：期刊论文
| 作者 | Xu, QJ; Huang, JX |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2000 |
| 卷号 | 28期号:1页码:46-49 |
| 关键词 | protein separation and purification ion-exchange column regeneration |
| 英文摘要 | Irreversible adsorption of proteins on high-performance ion-exchange packings can result in column deterioration and damage. TSKgel CM-5PW column and a DEAE-SPW (75 x 7.5 mm I.D.) column fouled by irreversible adsorption of proteins were treated with 2% trypsin solution under 37 degrees C for 24 hours, the back pressure was significantly declined, the number of theoretical plate was increased by 4 to 13 times and ion-exchange capacity was restored. The extent of column regeneration was related with the volume and concentration of the trypsin solution. |
| WOS记录号 | WOS:000085086800011 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/24146]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xu, QJ,Huang, JX. Regeneration of high performance ion exchange chromatographic column in protein separations and purifications[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(1):46-49. |
| APA | Xu, QJ,&Huang, JX.(2000).Regeneration of high performance ion exchange chromatographic column in protein separations and purifications.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(1),46-49. |
| MLA | Xu, QJ,et al."Regeneration of high performance ion exchange chromatographic column in protein separations and purifications".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.1(2000):46-49. |
入库方式： OAI收割
来源：生态环境研究中心
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