Chromatographic behavior of mouse serum IgG in protein G perfusion affinity chromatography
文献类型：期刊论文
| 作者 | Yan, Z; Huang, JX |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 1999 |
| 卷号 | 27期号:12页码:1365-1369 |
| 关键词 | immunoglobulin G perfusion chromatography affinity mass transfer |
| 英文摘要 | The mass transfer and chromatographic behavior of mouse serum immunoglobulin G(IgG) on protein G perfusion affinity chromatographic column were investigated experimentally. Experimental data have indicated that the protein G column had irreversible adsorption to IgG but no non-specific adsorption to the other proteins in serum under the investigation conditions. It was found that variations of the elution buffer composition and pH have played an essential effect to some extent on the retention time and the peak shape of IgG through changing the dissociation constant (K-D). The influence of mobile phase flow rate on the chromatographic behavior of IgG was also studied. The results indicated that the association/dissociation kinetics between IgG and protein G affinity packings became the rate-limiting step in perfusion affinity chromatographic separation process. |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000084530900001 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/24189]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yan, Z,Huang, JX. Chromatographic behavior of mouse serum IgG in protein G perfusion affinity chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,1999,27(12):1365-1369. |
| APA | Yan, Z,&Huang, JX.(1999).Chromatographic behavior of mouse serum IgG in protein G perfusion affinity chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,27(12),1365-1369. |
| MLA | Yan, Z,et al."Chromatographic behavior of mouse serum IgG in protein G perfusion affinity chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 27.12(1999):1365-1369. |
入库方式： OAI收割
来源：生态环境研究中心
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